TABLE 2.

Comparison of Anaplasma marginale subsp. centrale vaccine strain antigens with A. marginale sensu stricto orthologs

Israel vaccine strain protein annotationSt. Maries strain protein annotation% identitya
St. Maries strainFlorida strainMississippi strainPuerto Rico strainVirginia strain
Membrane proteins
    ACIS_00178 Omp7AM1220 Omp770, 10068, 8669, 9467, 9468, 99
    ACIS_00178 Omp7AM1221 Omp866, 10064, 9066, 9076, 10064, 88
    ACIS_00178 Omp7AM1222 Omp964, 10064, 99--64, 98
    ACIS_00140 Omp11AM1255 Omp1160, 10060, 9754, 8761, 97-
    ACIS_00138 Omp13AM1258 Omp1363, 10057, 9860, 9459, 97-
    ACIS_00068 Omp14AM075 Omp1471, 10071, 9773, 99-71, 97
    ACIS_00557 undefined productAM779 surface-expressed protein84, 10084, 10084, 9984, 10084, 100
    ACIS_00486 peptidoglycan-associated lipoproteinAM854 peptidoglycan-associated lipoprotein79, 10079, 10079, 10079, 10079, 100
    ACIS_00229 Msp2 N-terminal conserved regionAM1144 Msp2 N-terminal conserved region84, 10084, 9983, 9984, 10082, 98
    ACIS_00229 Msp2 C-terminal conserved regionAM1144 Msp2 C-terminal conserved region90, 10091, 10086, 9793, 9793, 97
    ACIS_00229 Msp2 hypervariable regionsAM1144 Msp2 hypervariable regions33-74b, 10036-67, 35-100b---
    ACIS_00617 Msp3 N-terminal conserved regionAM1063 Msp3 N-terminal conserved region52, 10051, 7455, 7749, 7753,77
    ACIS_00617 Msp3 C-terminal conserved regionAM1063 Msp3 C-terminal conserved region84, 10085, 9888, 9978, 9671, 88
    ACIS_00617 Msp3 hypervariable regionsAM1063 Msp3 hypervariable regions18-48b, 10013-44, 14-100b---
Housekeeping proteins
    ACIS_01060 succinyl-CoA synthetase (SucC)AM231 succinyl-CoA synthetase (SucC)98, 10099, 10097, 9998, 99-
    ACIS_01040 elongation factor Tu (TufB)AM254 elongation factor Tu (TufB)97, 10097, 10097, 10098, 10097, 100
    ACIS_00241 isocitrate dehydrogenase (Icd)AM1131 isocitrate dehydrogenase (Icd)99, 100100, 9999, 99100, 99100, 99
    ACIS_00394 chaperonin GroELAM944 60-kD chaperonin (GroEL)99, 10099, 10099, 10099, 10099, 100
    ACIS_00219 Atp synthase subunit alpha (AtpA)AM1174 F1-ATP synthase alpha subunit (AtpA)93, 10093, 10093, 10093, 9993, 100
  • a The St. Maries, Florida, Mississippi, Puerto Rico, and Virginia strains were compared to the Israel and St. Maries strains. The first number indicates sequence identity to the Israel strain, and the second number indicates sequence identity to the St. Maries strain. The Mississippi, Puerto Rico, and Virginia strain comparisons were performed using pyrosequencing data that comprise approximately 96% of the full sequence. Only partial sequences of ortholog gene products were available (12). A dash (-) indicates a partial sequence that does not allow comparison, not the absence of the gene.

  • b For Msp2 and Msp3 hypervariable regions, a range of sequence identities is given that represents the lowest and highest sequence identities observed when comparing all donor alleles in the genome.