TABLE 3.

Results of vaccination of BALB/c mice with recombinant Hcp proteins and challenge with 50 MLD of B. pseudomallei K96243

AntigenaNo. of survivors/ total vaccinatedBacteria present in the spleens of surviving animalsb
Animal 1Animal 2Animal 3Animal 4Animal 5
Hcp13/610300NAcNA
Hcp25/6>105>105102102102
Hcp33/6102103103NANA
Hcp42/6103103NANANA
Hcp50/5NANANANANA
Hcp63/6000NANA
Naïve0/6NANANANANA
SAS only0/6NANANANANA
  • a Recombinant Hcp proteins were mixed with SAS and delivered parentally three times over the course of a month. One mouse in the Hcp5 group died during vaccination, and so in that group only five animals were challenged with K96243.

  • b Spleens were removed from euthanized animals on day 42 and immersed in 1 ml of PBS and homogenized, and 100-μl aliquots were plated to estimate the total number of bacteria present.

  • c NA, not applicable.