Table 2.

Primers used in this study

No.NameSequence (5′–3′)aDescription
1N-lpl1545-BamHIATAGGATCCCCTCGTACAATGTTTTTTTCCGST-LegK1 overproduction
2C-lpl1545-SalIATAGTCGACTTACTCAGCCACTAACCATAAGGGST-LegK1 overproduction
3N-lpl2066-BamHIATAGGATCCGTTTATTACATAAATTTGAAGGAACGST-LegK2 overproduction
4C-lpl2066-SalIATAGTCGACTTAGCTTGGGCCTCGCATCGST-LegK2 overproduction
5N-lpl2481-BamHIATAGGATCCTTTGATAGAAATATAAAAGAAATAATCGST-LegK3 overproduction
6C-lpl2481-SalIATAGTCGACTTATAATTCAAAGCCTGAATGST-LegK3 overproduction
7N-lpl0262-BamHIATAGGATCCAAATTGCTTCGGTTTCATGAATT6His-LegK4 overproduction
8C-lpl0262-SalIATAGTCGACTTAATATGGCAAAATGATGACGT6His-LegK4 overproduction
9N-lpl2476-SacIATAGAGCTCGGGATTATCATGGCTACAGT6His-LegK5 overproduction
10C-lpl2476-PstIATACTGCAGTTATTTTATGAAATCGGCCTTTA6His-LegK5 overproduction
11Kan-FRTSCACGTCGACAGCGATTGTGTAGGCTGGAGCKm amplification
12Kan-FRTRGGGGATCCGTCGACCTGCKm amplification
13P1-dotA-NotIAAAAGCGGCCGCGCTCTCGCTGAAAGTGGCTClpl2613 deletion
14P2-dotA-SalICGCGTCGACTGCTTGCAAGCTCTTGGTTGlpl2613 deletion
15P3-dotA-SalICGCGTCGACGCCATTTCCTACATCCAATCGlpl2613 deletion
16P4-dotA-NotIAAAAGCGGCCGCCCGGTTTAGAGCTTGGTCCAlpl2613 deletion
17P1-legK1-NotIAAAAGCGGCCGCCGTTGATGCCGCTAATCTCClpl1545 deletion
18P2-legK1-SalICGCGTCGACCGGTTTAACCGCTATATGCCClpl1545 deletion
19P3-legK1-SalICGCGTCGACAAGGCTATCAAGCAGTTTTCCClpl1545 deletion
20P4-legK1-NotIAAAAGCGGCCGCTTTGAGAAAATAATCCCAGGCGlpl1545 deletion
21P1-legK2-NotIAAAAGCGGCCGCAATTTGAAGGAACAACCCTTACCTClpl2066 deletion
22P2-legK2-SalICGCGTCGACAAGTTTTTCCAGGACACATCCCTlpl2066 deletion
23P3-legK2-SalICGCGTCGACTTCGAATTTACAGGCTTACAAGGATClpl2066 deletion
24P4-legK2-NotIAAAAGCGGCCGCGCCTCGCATCAATGAAGGTGlpl2066 deletion
25P1-legK3-NotIAAAAGCGGCCGATTTACTTCCGGGCACTGGlpl2481 deletion
26P2-legK3-SalICGCGTCGACGGCATTCGTTTCATCGTCAGlpl2481 deletion
27P3-legK3-SalICGCGTCGACAGCAACTTGCGTCCATTACGlpl2481 deletion
28P4-legK3-NotIAAAAGCGGCCAGCTTCGCTTGCATGCAAAlpl2481 deletion
29P1-legK4-NotIAAAAGCGGCCGCAAGCCAATCATCGTTCCCAClpl0262 deletion
30P2-legK4-SalICGCGTCGACGTTCTGGTGCTAAATAGCTTGCGlpl0262 deletion
31P3-legK4-SalICGCGTCGACCTGCCACATCAAGTCCCCTClpl0262 deletion
32P4-legK4-NotIAAAAGCGGCCGCTGGCAAAATGATGACGTTGClpl0262 deletion
33P1-legK5-NotIAAAAGCGGCCGCCATGGCTACAGTAGATTCCGlpl2476 deletion
34P2-legK5-SalICGCGTCGACGAACGTTAGCTTCACGCTCTlpl2476 deletion
35P3-legK5-SalICGCGTCGACCACTGAAAATCGCGGATATCGlpl2476 deletion
36P4-legK5-NotIAAAAGCGGCCGCGTTCCATGTCAATTTTAGGGClpl2476 deletion
375-promolpl2066-SacIATAGAGCTCCAGGGTAACTGAATAAGCCClpl2066 complementation
383-promolpl2066-KpnICGGGGTACCTCTCCTACAAATCAATTGCClpl2066 complementation
395′SphI-promolpl2066ATAGCATGCCAGGGTAACTGAATAAGCCClpl2066 complementation
40lpl2066(K112M)-sensCCCAAAGAGAATATACTGATGGTTTTATATCAAAATTTTAGTAATGTCGlpl2066 mutagenesis
41lpl2066(K112M)-revCGACATTACTAAAATTTTGATATAAAACCATCAGTATATTCTCTTTGGGlpl2066 mutagenesis
  • a Restriction enzyme sites are indicated in boldface.