Table 2.

Selected agr-regulated genes in strains LAC, 252, and RN6390

Gene type and no.GeneFunctionFold change in gene expression (wild-type/agr strain)a
LAC252RN6390
Virulence
    SAUSA300_0113spaImmunoglobulin G binding protein A0.120.040.03
    SAUSA300_0256lrgAMurein hydrolase exporterNC0.35
    SAUSA300_0257lrgBMurein hydrolase export regulator0.09
    SAUSA300_0320gehLipase (EC 3.1.1.3)6.312.493.13
    SAUSA300_0424Low-affinity zinc transport protein7.33
    SAUSA300_0546sdrCNeurexin binding protein0.46
    SAUSA300_0547sdrDSurface binding protein0.17NP0.06
    SAUSA300_0548sdrESurface binding protein
    SAUSA300_0630Multidrug resistance ABC transporter7.5110.265.70
    SAUSA300_0772clfAClumping factor A (fibrinogen binding protein)2.082.57
    SAUSA300_0774empbpExtracellular matrix binding protein/fibrinogen binding protein0.36
    SAUSA300_0776nucThermonuclease (EC 3.1.31.1)0.180.27
    SAUSA300_0835dltAd-Alanine-activating enzyme (EC 6.3.2.-)0.330.46
    SAUSA300_0836dltBProtein DltB0.330.33
    SAUSA300_0837dltCd-Alanyl carrier protein0.430.41
    SAUSA300_0838dltDProtein DltD0.270.390.49
    SAUSA300_0949sspCHypothetical protein6.7927.33
    SAUSA300_0950sspBStaphopain (EC 3.4.22.-)9.7411.33
    SAUSA300_0951sspAGlutamyl endopeptidase (EC 3.4.21.19)11.309.59
    SAUSA300_1055efbFibrinogen binding protein3.00
    SAUSA300_1058hlaAlpha-hemolysin2.170.2523.68
    SAUSA300_1067psmβ1Phenol-soluble modulin beta 1173.3561.6141.55
    SAUSA300_1068psmβ2Phenol-soluble modulin beta 263.24NP26.07
    SAUSA300_1381lukF-PVLeukocidin F subunit7.14NPNP
    SAUSA300_1382lukS-PVLeukocidin S subunit6.52NPNP
    SAUSA300_1753splFSerine protease (EC 3.4.21.-)14.963.3289.05
    SAUSA300_1754splESerine protease (EC 3.4.21.-)11.1133.61
    SAUSA300_1755splDSerine protease (EC 3.4.21.-)10.8844.83
    SAUSA300_1756splCSerine protease (EC 3.4.21.-)9.9146.21
    SAUSA300_1757splBSerine protease (EC 3.4.21.-)10.7164.80
    SAUSA300_1758splASerine protease (EC 3.4.21.-)7.9927.45
    SAUSA300_1759Hypothetical protein69.53NC4.69
    SAUSA300_1918hlbSphingomyelin phosphodiesterase (EC 3.1.4.12), truncated beta-toxin6.398.85
    SAUSA300_1922sakStaphylokinase0.49
    SAUSA300_2440fnbBFibronectin binding protein0.24NP
    SAUSA300_2441fnbAFibronectin binding protein2.87
    SAUSA300_2603lipLipase (EC 3.1.1.3)12.547.5313.29
Resistance
    SAUSA300_0032mecAMecA protein4.70NC
    SAUSA300_0033mecR1Methicillin resistance protein3.02NC
    SAUSA300_0928comKCompetence transcription factor10.502.447.25
Metabolism
    SAUSA300_0061arcC1Carbamate kinase (EC 2.7.2.2) (ACME)NPNC
    SAUSA300_0062arcB1Ornithine carbamoyltransferase (EC 2.1.3.3) (ACME)0.46NPNC
    SAUSA300_0063Transcription regulator, crp family (ACME)0.45NPNC
    SAUSA300_0064arcD1Arginine/ornithine antiporter (ACME)0.40NPNC
    SAUSA300_0065arcA1Arginine deiminase (EC 3.5.3.6) (ACME)0.41NPNC
    SAUSA300_0220pflBFormate acetyltransferase (EC 2.3.1.54)0.10
    SAUSA300_0221pflAPyruvate formate-lyase-activating enzyme (EC 1.97.1.4)0.19
    SAUSA300_0311Ribokinase (EC 2.7.1.15)0.050.060.16
    SAUSA300_0312Sugar kinase0.050.040.26
    SAUSA300_0313Nucleoside permease nupC0.050.050.19
    SAUSA300_0863argHArgininosuccinate lyase (EC 4.3.2.1)58.19
    SAUSA300_0864argGArgininosuccinate synthase (EC 6.3.4.5)54.46
    SAUSA300_1062argFOrnithine carbamoyltransferase (EC 2.1.3.3)0.070.120.31
    SAUSA300_1063arcC3Carbamate kinase (EC 2.7.2.2)0.080.190.30
    SAUSA300_1712ribH6,7-Dimethyl-8-ribityllumazine synthase (EC 2.5.1.9)31.50
    SAUSA300_1713ribBAGTP cyclohydrolase II (EC 3.5.4.25)/3,4-dihydroxy-2-butanone-4-phosphate synthase (EC 4.1.2.-)15.58
    SAUSA300_1714ribERiboflavin synthase alpha chain (EC 2.5.1.9)42.18
    SAUSA300_1715ribDDiaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26)/5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)24.48
    SAUSA300_2567arcC2Carbamate kinase (EC 2.7.2.2)0.150.08
    SAUSA300_2568arcD2Arginine/ornithine antiporter0.150.21
    SAUSA300_2569arcB2Ornithine carbamoyltransferase (EC 2.1.3.3)0.180.11
    SAUSA300_2570arcA2Arginine deiminase (EC 3.5.3.6)0.220.05
Regulation
    SAUSA300_0605sarAStaphylococcal accessory regulator2.77
    SAUSA300_0690saeSSensory transduction protein kinase (EC 2.7.3.-)2.61
    SAUSA300_0691saeRTwo-component response regulator2.17
    SAUSA300_1708rotStaphylococcal accessory regulator0.643.18
Transport
    SAUSA300_2453ABC transporter ATP binding protein0.050.090.20
    SAUSA300_2454ABC transporter ATP binding protein0.060.060.15
  • a NC, empty fields, no significant change; NP, gene not present in that strain.