Table 1

Summary of data used for comparative evaluation of 8 predicted T-cell epitopes of Pep1, Amn1, and Plb antigensa

Synthetic epitope peptideSequence of computationally predicted epitope peptidesProPred binding prediction (% of alleles)ELISPOT assay (no. of spots per well)DELFIA (IC50 [nM])b% sequence similarity between speciescHomology to human proteins (E-value)dNo. of nonsynonymous coding SNPe
Pep1-P1MRNSILLAATVLLGCTSAKVHL10012586.110010−20
Pep1-P2HVRALGQKYFGSLPSSQQQTV8510064.310010−10
Amn1-P9LFETTIRYLGGMISAYDLLK1001251,649.110010−60
Amn1-P10PAKVDVLLAQSLKLADVLKF100100322.810010−10
Amn1-P11NGLATTGTLVLEWTRLSDIT100100166.610010−20
Amn1-P13YYNLRPEVIESIYYAYRMTK92501,826.710010−50
Plb-P2AIPLDSNVHIRALPNAPNGY901752,954.49510−21
Plb-P6TPLVVYIPNYPYTTWSNIST90125815.69510−31
  • a The synthetic epitope peptides listed are those predicted to bind to >80% of 51 human MHC II alleles in the ProPred algorithm. The same peptides were also shown to stimulate IFN-γ production by immune CD4+ T cells (measured by ELISPOT assays) as previously reported (46, 47). The synthetic peptides indicated in bold type were ultimately selected for incorporation into an epitope-based vaccine construct.

  • b Peptide concentrations that showed 50% inhibition of CLIP binding to HLA-DR4 transgenic mouse-derived major histocompatibility class II (MHC II) molecules in the DELFIA.

  • c Results of alignment of peptide sequences of C. immitis and C. posadasii isolates using the Broad Institute Coccidioides Group database.

  • d E-values of the results of an NCBI BLASTP search of peptide sequence similarities (1) with proteins in the Homo sapiens protein database.

  • e Numbers of nonsynonymous coding single-nucleotide polymorphisms (SNP) identified for each peptide sequence (37).