Table 3

Concentrations (pg/ml) of cytokines secreted in vitro by rEBV- versus OVA-stimulated immune splenocytes isolated from nonvaccinated mice versus mice vaccinated with rEBV plus GP plus OVA and sacrificed at 7 days postchallengea

Secreted cytokineIn vitro stimulation of immune splenocytes from nonvaccinated and vaccinated mice
OVA (1 μg/ml)rEBV (1 μg/ml)OVA (10 μg/ml)rEBV (10 μg/ml)
NonvaccinatedVaccinatedNonvaccinatedVaccinatedNonvaccinatedVaccinatedNonvaccinatedVaccinated
Proinflammatory
    IL-6110.3 ± 7.174.5 ± 2.61,614.1 ± 54.93,391.6 ± 141.5*192.4 ± 9.697.1 ± 3.32,004.2 ± 88.32,925.2 ± 213*
    IL-17A404.9 ± 41.7160.7 ± 2.8258.5 ± 13.21,317.1 ± 73.6*974.6 ± 21.8331.4 ± 29.9153.4 ± 6.61,460.7 ± 99.3*
Regulatory
    IL-1026.8 ± 2.329.4 ± 1.5102.1 ± 1.3190.9 ± 16.9*36.5 ± 2.130.8 ± 1.9416.2 ± 6.7803.1 ± 10.1*
Th1 type
    IL-2270.6 ± 11.0231.7 ± 3.532.0 ± 2.5450.4 ± 11.3*462.6 ± 20.0270.6 ± 1.332.9 ± 2.4553.4 ± 28.9*
    IFN-γ91.5 ± 6.619.3 ± 3.12,138.1 ± 189.64,880.4 ± 698.5*217.2 ± 8.423.6 ± 4.51,853.5 ± 3404,163.7 ± 278*
Th2 Type
    IL-428.3 ± 0.712.7 ± 0.19.0 ± 0.345.2 ± 1.9*45.0 ± 2.215.0 ± 0.44.0 ± 0.158.3 ± 1.2*
    IL-5127.3 ± 4.244.2 ± 1.218.3 ± 1.6176.0 ± 5.7*208.1 ± 13.374.3 ± 0.914.9 ± 1.4161.4 ± 6.2*
  • a OVA or rEBV protein was added to immune cells in growth medium and the mixture was incubated for 48 h. Nonvaccinated mice were immunized with GP plus OVA alone. Asterisks indicate a significant difference between cytokine concentrations (P < 0.05) in culture supernatants of rEBV-stimulated immune splenocytes derived from nonvaccinated versus vaccinated mice.