Table 1

Characteristics of 61 sequence type ST131 and non-ST131 extraintestinal E. coli clinical isolates studied in the murine sepsis model

Study groupStrain identification no.Epidemiological backgroundPhylogenetic backgroundResistance traitVirulence traitMouse result
YearaLocaleClinical contextbST/CC (O:K:H serotype)cPhylodESBLeFQRfVF scoregExPEChLethalityiMean statusj
ST131 (FQ-R)JJ18992004Galveston, TXPost-renal transplant bacteriuriaST131B2Yes8.00Yes0.102.00
JJ19042005Galveston, TXPost-renal transplant bacteriuriaST131B2Yes9.00Yes0.001.33
JMI-1852009Louisville, KYBacteremiaST131B2YesYes12.00Yes0.001.67
JMI-2682006Little Rock, ARn/akST131B2Yes11.00Yes1.005.00
SaT-0492007Stamford, CTWoundST131B2Yes9.00Yes0.001.00
SaT-1582003New York, NYBacteremiaST131B2Yes9.00Yes0.001.00
Z-1642004Winnipeg, MBCystitisST131B2Yes9.00Yes0.202.00
Z-1932004Moncton, NBCystitisST131B2Yes10.00Yes1.004.67
MV00842010Minneapolis, MNAsymptomatic bacteriuriaST131B2Yes8.00Yes0.201.50
MV01582010Minneapolis, MNAsymptomatic bacteriuriaST131B2Yes10.00Yes0.301.83
MV01792011Minneapolis, MNFebrile urinary tract infectionST131B2Yes9.00Yes1.004.67
ST131 (FQ-S)JJ18972004Galveston, TXPost-renal transplant bacteriuriaST131B211.00Yes1.004.17
JJ19012004Galveston, TXPost-renal transplant bacteriuriaST131B212.00Yes0.503.00
JMI-1782006Little Rock, ARBacteremiaST131B210.00Yes0.001.67
JMI-2372009Louisville, KYBacteremiaST131B29.00Yes1.004.67
SaT-0402007Burlington, VTBacteriuriaST131B212.00Yes0.202.00
SaT-1422003New York, NYn/aST131B212.00Yes1.004.67
Z-0632002Winnipeg, MBCystitisST131B212.00Yes1.003.67
Z-0712002Moncton, NBCystitisST131B210.00Yes1.004.33
MV01302010Minneapolis, MNCystitisST131B212.00Yes0.302.00
MV01312010Minneapolis, MNAsymptomatic bacteriuriaST131B28.00Yes0.002.00
MV01672011Minneapolis, MNBacteriuriaST131B28.00Yes0.001.33
Non-ST131 (FQ-R)JJ21062004Galveston, TXPost-renal transplant bacteriuriaNon-ST131AYes3.00Yes0.001.33
JMI-1722006Kansas City, MOBacteremiaNon-ST131B2Yes12.75Yes0.101.33
SaT-0342007Burlington, VTBacteriuriaNon-ST131B2Yes13.00Yes0.402.50
Z-0642002Winnipeg, MBCystitisNon-ST131B2Yes8.00Yes1.004.67
JJ21122005Galveston, TXPost-renal transplant bacteriuriaNon-ST131DYes5.000.001.33
JMI-2162009Louisville, KYBacteremiaNon-ST131DYes6.00Yes0.403.00
SaT-1572003New York, NYBacteriuriaNon-ST131DYes7.00Yes1.005.00
Z-1362004Moncton, NBCystitisNon-ST131DYes8.00Yes1.004.67
MV00372010Minneapolis, MNUrosepsisNon-ST131DYes6.00Yes1.004.67
MV00952010Minneapolis, MNUrosepsisNon-ST131DYes6.25Yes0.001.33
MV01172010Minneapolis, MNUrinary tract infectionNon-ST131DYes8.00Yes0.101.50
Non-ST131 (FQ-S)JJ21112005Galveston, TXPost-renal transplant bacteriuriaNon-ST131A8.00Yes0.704.00
JMI-2352009Lexington, KYBacteremiaNon-ST131A2.000.001.67
Z-0042002Winnipeg, MBCystitisNon-ST131A7.25Yes0.002.00
SaT-0382007Burlington, VTBacteriuriaNon-ST131B13.000.001.00
Z-0112002Moncton, NBCystitisNon-ST131B18.00Yes1.003.67
JJ21012004Galveston, TXPost-renal transplant bacteriuriaNon-ST131B212.00Yes1.004.67
MV00092010Minneapolis, MNPyelonephritisNon-ST131B217.00Yes1.004.67
MV00212010Minneapolis, MNSurgical wound infectionNon-ST131B213.00Yes0.002.00
MV01122010Minneapolis, MNCystitisNon-ST131B212.50Yes1.004.67
JMI-1452006Little Rock, ARBacteremiaNon-ST131D6.00Yes0.903.67
SaT-1562003New York, NYBacteremiaNon-ST131DYes10.00Yes0.001.33
Classic ExPEC536n/an/aPyelonephritisST127 (O6:K15:H31)B211.00Yes1.005.00
CFT073n/an/aPyelonephritis with bacteremiaST73 (O6:K2:H1)B217.00Yes0.744.00
ECOR 64n/an/aCystitisST81 (O75:K5:H−)B211.00Yes0.402.67
NU14n/an/aCystitisST95 (O18:K1:H7)B217.00Yes0.001.33
RS218n/an/aNeonatal meningitisST95 (O18:K1:H7)B217.00Yes1.005.00
U8n/an/aCystitisST95 (O18:K1:H7)B217.00Yes1.005.00
IA2n/an/aUrinary tract infectionST12/CC12 (O4:K12:H−)B215.00Yes0.003.00
CP9n/an/aBacteremiaST599/CC12 (O4:K54:H5)B215.00Yes1.005.00
PY76n/an/aPyelonephritisST69 (O17:K52:H18)D9.00Yes0.211.67
2P9n/an/aUrosepsisST598/CC31 (O15:K52:H1)D8.00Yes0.904.67
PY14n/an/aPyelonephritisST59 (O1:K1:H−)D10.00Yes0.202.67
ECOR 40n/an/aPyelonephritisST62 (O7:K1:H−)D6.00Yes0.804.00
ST131 case reportsCU7582007Columbia, MOSeptic arthritis, osteomyelitisST131B2Yes10.00Yes1.005.00
JJ18862008Portland, MEFatal urosepsis (see JJ1887)ST131B2YesYes9.00Yes0.704.67
JJ18872008Portland, MERecurrent cystitis (see JJ1886)ST131B2YesYes9.00Yes0.503.17
JJ25462008Bethlehem, PARenal abscesses (see JJ2547)ST131B2YesYes11.00Yes0.402.33
JJ25472008Bethlehem, PAPyelonephritis (see JJ2546)ST131B2YesYes11.00Yes0.001.67
  • a Year, year of isolation (shown only for recent clinical isolates).

  • b Clinical context based on patient's clinical presentation, if known; otherwise, on specimen type. Paired same-household isolates JJ1886 and JJ1887 and JJ2546 and JJ2547 involved presumed within-household transmission, as did CU758 (which matched a fecal isolate from the 4-month-old patient's mother that was not studied here).

  • c CC, clonal complex (= ST complex); includes STs that differ from the complex's index ST at a single locus. O:K:H serotype shown only for classic extraintestinal pathogenic E. coli (ExPEC) isolates.

  • d Phylo, major phylogenetic group (A, B1, B2, D).

  • e ESBL, extended-spectrum β-lactamase production. “No” is indicated by blank cells.

  • f FQR, fluoroquinolone resistance. “No” is indicated by blank cells.

  • g VF score, virulence factor score; calculated as the sum of all virulence genes detected, with adjustment for multiple detection of the pap (P fimbriae), sfa/foc (S and F1C fimbriae), and kpsM II (group 2 capsule) operons.

  • h ExPEC, extraintestinal pathogenic E. coli, defined as presence of ≥2 of papAH and/or papC (P fimbriae structural subunit and assembly), sfa/foc, afa/dra (Dr family adhesins), iutA (aerobactin system), and kpsM II. “No” is indicated by blank cells.

  • i Lethality, fraction of mice challenged with isolate (among ≥5 mice per isolate) that became moribund or died by 3 days postchallenge.

  • j Median value for mouse mean status (according to daily observations over 3 days postchallenge), among the ≥5 mice challenged per isolate, where 1 = healthy, 2 = mildly ill, 3 = moderately ill, 4 = severely ill/moribund, and 5 = dead.

  • k n/a, not available or not applicable.