Table 1

Primers used in the study

PrimerSequence (5′–3′)a
com_ompC-2 (HindIII)CCCAAGCTTCAACAGTAAAAAGAAGCGGG
com_ompC-2 (XbaI)GCTCTAGACAACAGTAAAAAGAAGCGGG
dpompC (SspI+StuI)ACCTAATATTAGGCCTTTTTTATGCCACTGCATACTG
dsompC (XbaI)GCTCTAGAATTGCTGATGCGACTGATTG
dsompC (SspI+StuI)CCTAATATTAGGCCTGTGGCTACGACGACGAAGAT
com_ompC-1 (StuI)AAGGCCTGCGGGTTGTGGTTTTTGATC
com_ompC-2 (StuI)AAGGCCTCAACAGTAAAAAGAAGCGGG
rfOmpC-1 (BamHI)CGGGATCCGATGAAAGTTAAAGTACTGTC
rfOmpC-2 (XhoI)CCCGCTCGAGTTAGAACTGGTAAACCAGAC
  • a Introduced restriction enzyme sites are underlined.