Table 2

Plaque sizes of LLO domain 1 mutants

Mutated residueaPlaque sizeb
Y126A65.0 ± 5.4**
R149A14.8 ± 2.8***
K175A44.1 ± 1.1***
L185A47.5 ± 1.5***
Y197A18.1 ± 3.0*
Y255A66.7 ± 4.7**
Y256A8.1 ± 0.3****
F269A33.7 ± 4.3**
F270A47.8 ± 4.0**
L279A44.3 ± 4.2**
Y292A49.8 ± 5.9**
Y298A21.6 ± 8.1**
  • a Each mutant strain was analyzed in at least three experiments.

  • b Diameters of bacterial plaques is expressed as a percentage of the diameter of wild-type plaques and includes the standard deviation. Student's t test was used to analyze statistical significance versus wild-type bacteria.

  • * , P < 0.05;

  • ** , P < 0.01;

  • *** , P < 0.001;

  • **** , P < 0.0001.