Table 2

Properties of the murine anti-fHbp MAbs

Mouse MAb designation (Ig subclass)Variant groups recognizedaModular group recognizedbEpitope amino acid position(s)cBactericidal activitydReference(s)
JAR 4 (G2a)1, 2I, III, VID25, K27, YGN (57–59)YesWelsh et al. (60), Beernink et al. (6)
JAR 31 (G2b)2, 3II, III, V, VID201YesPajon et al. (42)
JAR 33 (G2a)2, 3II, IIIR180, E192YesBeernink et al. (10)
JAR 35 (G2b)2, 3II, IIIK174YesBeernink et al. (10)
JAR 41 (G1)1, 2, 3AllD25YesVu et al. (53)
JAR 64 (G1)1, 2, 3AllUnknowneNoDavid Vu, unpublished
  • a According to fHbp classification by Masignani et al. (38).

  • b According to classification by Beernink and Granoff (4, 42); only the six prevalent modular groups are listed.

  • c Amino acid substitutions knocked out and/or knocked in expression of the epitope (6, 10).

  • d Cooperative bactericidal activity with human complement and second anti-fHbp MAbs that individually were not bactericidal (6, 10, 59, 60). All six MAbs bound to the surface of live encapsulated strains as measured by flow cytometry.

  • e Epitope overlaps that of JAR 41, based on inhibition of binding of JAR 41 to fHbp (David Vu, unpublished data).