Table 1

Bacterial strains used in this work

StrainGenotype or descriptionSource
E. coli strain
    DH5αfhuA2 Δ(argF-lacZ)U169 phoA glnV44 ϕ80dlacZΔM15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17Invitrogen
M. tuberculosis strains
    H37RvPHRI collectiona
    TB15Η37Rv derivative; ΔwhiB5 hyg (whiB5 mutant)This work
    TB16ΤΒ15 derivative containing pSC25; ΔwhiB5::whiB5 hyg Kmr (whiB5-complemented strain)This work
    TB60H37Rv derivative containing pMYT769; PfurA-pip int oriE Smr (strain containing empty pristinamycin-inducible system)This work
    TB84H37Rv derivative containing pMYT790; Pptr-whiB5 PfurA-pip int oriE Smr (strain expressing whiB5 from the pristinamycin-inducible system)This work
    TB141H37Rv derivative containing pSC43 (strain expressing GFP from the whiB5 promoter region)This work
    TB142TB15 derivative containing pSC43 (strain expressing GFP from the whiB5 promoter region)This work
  • a PHRI, Public Health Research Institute.