Table 1

qRT-PCR data for selected genes identified as significantly changed or unchanged in expression at 2 and 24 h postinfection in bladder during GBS cystitis according to microarrays

Target geneaTime (h)Mean CT ± SEM (n = 5)ΔCTFold changePb
ControlInfected
CXCL10234.111 ± 1.2933.351 ± 0.530.751.4110.110
2435.208 ± 3.1428.019 ± 6.827.18930.750.001
iNOS236.73 ± 1.2536.75 ± 2.02−0.02−1.0820.817
2435.73 ± 0.4432.26 ± 2.813.4711.920.001
CCL5228.37 ± 1.4028.425 ± 1.02−0.0551.40.153
2429.192 ± 1.1528.015 ± 1.211.1772.5250.003
CXCL5235.34 ± 0.8735.21 ± 2.450.131.130.717
2435.64 ± 0.5633.73 ± 1.801.914.960.001
CXCL9235.573 ± 0.8134.213 ± 3.571.361.7770.075
2436.753 ± 1.3231.950 ± 2.784.8036.4640.008
IL-1α231.717 ± 1.0530.737 ± 3.190.982.7880.001
2432.655 ± 0.9529.012 ± 2.053.64322.110.001
IL-1β227.766 ± 1.1125.343 ± 1.872.4237.580.001
2429.455 ± 2.3524.013 ± 3.155.44221.0960.001
IL-6233.47 ± 5.7030.77 ± 10.92.57.9130.001
2437.03 ± 1.3433.07 ± 6.223.1417.940.001
EMR1228.06 ± 0.1228.40 ± 0.380.41.0720.824
2434.48 ± 2.6833.14 ± 1.021.342.5210.124
CSF1R222.16 ± 0.0622.60 ± 0.020.441.510.396
2428.02 ± 1.327.22 ± 0.690.81.730.199
INFGr1233.097 ± 0.3333.110 ± 0.62−0.0131.1030.539
2435.340 ± 3.6833.477 ± 0.621.8631.120.480
Caspase-3230.91 ± 0.6331.25 ± 1.17−0.34−1.0890.724
2430.92 ± 1.2730.64 ± 1.110.281.2930.3
β-Actin219.938 ± 0.2220.256 ± 0.13−0.3180.8150.113
2420.26 ± 0.3120.36 ± 0.15−0.10.9370.127
GAPDH220.162 ± 0.320.662 ± 0.43−0.5−1.180.5
2420.531 ± 0.2320.352 ± 0.250.1791.1320.658
  • a Primer sequences are listed in Table S1 in the supplemental material.

  • b P values compare GBS-infected group with control (PBS) group within each time point per Materials and Methods.