Table 1

Bacterial strains used in this study

Strain or plasmidGenotype or descriptionReference or source
Strains
    Escherichia coli
        BW25141Cloning strain9
        ER2566Overexpression strainNew England Biolabs
        AH-pfsER2566/pET28a-pfs (Kanr)This work
        AH-luxSER2566/pET28a-luxS (Kanr)This work
        AH1070ER2566/pET28a-lsrB (Kanr)This work
    Yersinia pestis
        CO92Yersinia pestis Lcr+ Pgm+7
        R88CO92 Lcr+ ΔpgmR. D. Perry
        R115CO92 Lcr+ Δpgm ΔypeIR ΔyspIR ΔluxS::kanR. D. Perry
        ISM1980R88 ΔluxS::kanThis work
        YP21R88 ΔluxS::kanThis work
        YP23R88 ΔluxS::frt/pJET1.2 (Ampr)This work
        YP21R88 ΔluxS::frt/pJET1.2-luxSThis work
        YP24R88 Δlsr::frtThis work
    Vibrio harveyi MM32luxN::cat luxS::Tn5kan12
Plasmids
    pET28aOverexpression vector (Kanr)Novagen
    pET28a-pfsPfs expression vector (Kanr)This work
    pET28a-luxSLuxS expression vector (Kanr)This work
    pET28a-lsrBLsrB(Δ2–25) expression vector (Kanr)This work
    pJET1.2Cloning vector (Ampr)Fermentas
    pJET1.2-luxSluxS complementation vector (Ampr)This work