Table 1

Properties of VHHs recognizing Stx1 and/or Stx2

VHH nameCloneProteinSpecificitySubunitaKD (nM) forb:Neutralizing activity (nM) against:GenBank accession no.
Stx1Stx2
Stx1Stx2EC50cIC50dEC50cIC50d
Stx1-A9JFA-26JET-A9Stx1B7.6 ± 0.9NB1010>1,000>1,000KF551949
Stx1-D4JGL-8JGG-D4Stx1A0.128 ± 0.006NB0.5>1,000>1,000>1,000KF551950
Stx-A4JFL-17JFD-A4Stx1/Stx2B7.2 ± 0.812 ± 430>3301050KF551951
Stx-A5JFL-29JFD-A5Stx1/Stx2B12.5 ± 0.97.7 ± 0.515100110KF551952
Stx2-A6JFA-31JEU-A6Stx2BNB5 ± 2>1,000ND15KF551953
Stx2-D2JFA-36JEU-D2Stx2eBNB7.0 ± 0.9>1000ND220KF551954
Stx2-D10JFL-47JEN-D10Stx2BNB0.21 ± 0.01>1000>1,0000.30.7KF551955
Stx2-G1JGL-34JGH-G1Stx2BNB0.023 ± 0.003>1,000>1,0000.10.04KF551956
Stx2-G9JGL-40JGH-G9Stx2eBNB19 ± 2>1,000>1,00023KF551957
Stx2-H6JFL-88JFG-H6Stx2BNB0.41 ± 0.01>1,000>1,0000.51KF551958
  • a Subunit assessed by Western blotting.

  • b KD assessed by SPR; mean ± SD. NB, no significant binding was detected.

  • c EC50s assessed by dilution ELISAs (see Fig. 2 and 3).

  • d IC50s assessed by cell assays (see Fig. 4 and 5). ND, not done.

  • e Slight cross-reactivity to Stx1.