Table 2

Properties of VHH heteromultimers recognizing Stx1 and/or Stx2

Heteromultimer nameCloneSpecificityKD (nM)a for:Neutralizing activity (nM) against:
Stx1Stx2
VHH 1VHH 2VHH 3Stx1Stx2EC50bIC50cEC50bIC50c
A5/A4JFX-10Stx-A5Stx-A4None0.74 ± 0.040.9 ± 0.10.40.30.30.05
A9/A4JFX-27Stx1-A9Stx-A4None0.50 ± 0.0380 ± 200.50.0550>100
A9/D4JGX-2Stx1-A9Stx1-D4None1.2 ± 0.4NB0.61ND>100
A5/D10JFX-16Stx-A5Stx2-D10None9.2 ± 0.80.20 ± 0.0130500.80.02
G1/D10JGX-19Stx2-G1Stx2-D10NoneNB0.004 ± 0.005>1,000>1000.30.04
A9/A5/D10JFZ-29Stx1-A9Stx-A5Stx2-D100.71 ± 0.030.7 ± 0.10.30.080.30.03
A9/A5/G1JHO-2Stx1-A9Stx-A5Stx2-G10.46 ± 0.020.09 ± 0.020.50.050.30.04
  • a KD assessed by SPR; mean ± SD. NB, no significant binding was detected.

  • b EC50s assessed by dilution ELISAs (see Fig. 2 and 3). ND, not done.

  • c IC50s assessed by cell assays (see Fig. 4 and 5).