Table 2

Transposon mutants with reduced NaCl-induced colistin resistance

Function(s) and strainInsertion siteLocus tagbGene annotation
Phosphate metabolism
    2F12IntergenicA1S_2445High-affinity phosphate transport protein (PstB)
    2F12IntergenicA1S_2444Putative periplasmic protease
    19H7GeneA1S_3030Phosphate-inducible protein, PhoH-like
    22F11GeneA1S_0462Putative phosphatase
Envelope biogenesis
    6D2GeneA1S_2982YidD
    11E4GeneA1S_2250Zn-dependent protease with chaperone function
    20A5IntergenicA1S_3424Putative lipoprotein-34 precursor (NlpB)
    5A7GeneA1S_0430Putative glycosyltransferase (LpsB)
    23E5GeneA1S_1030DNA-binding ATP-dependent protease La
    19C4GeneA1S_0499Putative Fe-S cluster redox enzyme (rRNA large-subunit methyltransferase N)
RNA synthesis, processing, modification, and degradation
    20A5IntergenicA1S_3425Phosphoribosylaminoimidazole-succinocarboxamide
    18G3aIntergenicA1S_0531Putative GTPase
    18G3aIntergenicA1S_0532Oligoribonuclease
    19C4GeneA1S_0499Putative Fe-S cluster redox enzyme (rRNA large-subunit methyltransferase N)
    6D2GeneA1S_2982YidD
Amino acid transport and metabolism (glutamate, aspartate, alanine), urea cycle
    22E8GeneA1S_3185Glutamate synthase subunit alpha
    17H6IntergenicA1S_1142Aspartate kinase
    17H6IntergenicA1S_1143Hypothetical protein
    20B10GeneA1S_2793Putative amino acid transport protein
    41C12aGeneA1S_2454Diaminobutyrate-2-oxoglutarate transaminase
    20A2IntergenicA1S_2023Hypothetical protein
    20A2IntergenicA1S_2024Glutamate 5-kinase
    20A1GeneA1S_3025Malate dehydrogenase
TCA cycle
    31A2GeneA1S_2710Citrate synthase
Cofactor biosynthesis
    14G3aGeneA1S_08078-Amino-7-oxononanoate synthase
Multidrug efflux system
    18D2GeneA1S_0909Multidrug resistance protein B
    20A11IntergenicA1S_1231Major facilitator superfamily transporter
    20A11IntergenicA1S_1232EsvB
Lipid metabolism
    3H3IntergenicA1S_2842Putative acetyl-CoA acetyltransferase
    20B2aIntergenicA1S_3159Lipase chaperone
    20B2aIntergenicA1S_3160Lipase
Phage
    37A5aIntergenicA1S_1585Putative replicative DNA helicase
    37A5aIntergenicA1S_1586EsvKI
  • a Mutant that is more resistant to colistin.

  • b Locus tags refer to the NCBI reference sequence NC_009085.