Table 1

Bacterial strains and plasmids

Strain or plasmidDescriptionSource or reference(s)
Wild-type strains
    UTI89UPEC, cystitis isolate (O18:K1:H7)6, 95
    CFT073Urosepsis isolate (O6:K2:H1)96
Recombinant strains
    UTI89ΔcpxPUTI89 ΔcpxP::clmrThis study
    UTI89ΔcpxRAUTI89 ΔcpxRA::clmrThis study
    UTI89ΔfieFUTI89 ΔfieF::clmrThis study
    CFT073ΔcpxPCFT073 ΔcpxP::clmrThis study
    CFT073ΔcpxRACFT073 ΔcpxRA::clmrThis study
    CFT073ΔfieFCFT073 ΔfieF::clmrThis study
Plasmids
    pKM208IPTG-inducible Red recombinase expression plasmid, Ampr60
    pGEN-MCSHigh-retention plasmid containing empty multiple-cloning site, Ampr61
    pJLJ41pcpxP sequence with native promoter from UTI89 cloned into pGEN-MCS; AmprThis study
    pJLJ42cpxRA sequence with native promoter from UTI89 cloned into pGEN-MCS; AmprThis study
    pNLP10-luxLow-copy-number cloning vector with promoterless luxCDABE operon; Kanr39
    pJW1-cpxP-luxpNLP10 with PcpxP::luxCDABE, Kanr39