Table 2

Primers used in this studya

PrimerSequence (5′–3′)
cpxP KO
    ForwardATGCGCATAGTTACCGCTGCCGTCATGGCCTCAACGCTGGGTGTAGGCTGGAGCTGCTTC
    ReverseCTACTGGGAACGTGAGTTGCTACTACTCAATAGCTTCAACCATATGAATATCCTCCTTAG
cpxP confirmation
    ForwardCTATCGTTGAATCGCGACAG
    ReverseGGATGGTGTCTATGGCAAGG
cpxRA KO
    ForwardATGAATAAAATCCTGTTAGTTGATGATGACCGAGAGCTGGTGTAGGCTGGAGCTGCTTC
    ReverseTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTCATATGAATATCCTCCTTAG
cpxRA confirmation
    ForwardACTGCCAGCGTTGAGGCCATGA
    ReverseGAGTGTAGGCCTGATAAGACGCTATCAGC
fieF KO
    ForwardATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCTGTGTAGGCTGGAGCTGCTTCG
    ReverseTTATGAAAGCATAGACCGTTTACCCTCCCTGGGTACGACGCATATGAATATCCTCCTTAG
fieF confirmation
    ForwardCCTTGCCATAGACACCATC
    ReverseTCAGGTCAGGCCAAATGG
pJLJ41p
    Forward (PstI)AATCCTGCAGATTGTTTAAATACCTCCGAGGC
    Reverse (SalI)TAGAGTCGACTACCAGCGCGGCGAGAATAC
pJLJ42
    Forward (PstI)TGCTCTGCAGTCATTTGCTCCCAAAATCTTTCT
    Reverse (SalI)GCTAGTCGACAGCGGCAAGATCGAAGATTTTT
  • a Added restriction sites underlined. KO, knockout.