TABLE 1

Strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
L. pneumophila strains
    MB110Strain Lp02; thyA rpsL hsdR20
    MB670thyA+ rpsL hsdRThis study
    MB698MB110/pMMB206ΔmobLaboratory collection
    MB703thyA rpsL hsdR phtC::kanThis study
    MB704thyA rpsL hsdR phtD::GentThis study
    MB705thyA rpsL hsdR phtD::kanThis study
    MB706MB110/pphtCThis study
    MB707MB110/pphtDThis study
    MB708MB703/pMMB206ΔmobThis study
    MB709MB703/pphtCThis study
    MB710MB703/pphtDThis study
    MB711MB705/pMMB206ΔmobThis study
    MB712MB705/pphtCThis study
    MB713MB705/pphtDThis study
    MB717thyA+ rpsL hsdR phtC::kanThis study
    MB718thyA+ rpsL hsdR phtD::kanThis study
    MB720MB670/pMMB206ΔmobThis study
    MB723MB717/pMMB206ΔmobThis study
    MB724MB717/pphtCThis study
    MB725MB717/pphtDThis study
    MB1338MB703/pKCDBThis study
    MB1339MB704/pKCDBThis study
    MB1342MB110/pSU2719
    SØ6687araD139 ΔlacU169 strA thi codAB ΔnupC ΔnupG ΔxapB::Kanr34
    GD1381SØ6687/pGD25734
Plasmids
    pGEM-TeasyCloning vector; AprPromega
    pUC4KSource of the1.3-kb kanamycin resistance cassette; KmrGE Life Sciences
    pGEMT-GentSource of the 1.7-kb gentamicin resistance cassette; Apr Gentr24
    pMMB206ΔmobΔmob lacIq Ptac lacUV5; derivative of plasmid pMMB206 in which the mobilization potential functions have been deleted; Cmr13
    pGEM-phtCA 1.0-kb PCR fragment of the phtC gene ligated into plasmid pGEM-Teasy; AprThis study
    pGEM-phtDA 1.0-kb PCR fragment of the phtD gene ligated into plasmid pGEM-Teasy; AprThis study
    pGEM-phtC::kanThe 1.3-kb kanamycin resistance cassette amplified from pUC4K was ligated into pGEM-phtC linearized with StuI; Apr KmrThis study
    pGEM-phtC::GentEcoRI-digested gentamicin resistance cassette from pGEMT-Gent ligated into pGEM-phtC digested with StuI; Apr GentrThis study
    pGEM-phtD::kanThe 1.3-kb kanamycin resistance cassette amplified from pUC4K was ligated into pGEM-phtD linearized with EcoRV; Apr KmrThis study
    pGEM-phtD::GentEcoRI-digested gentamicin resistance cassette from pGEMT-Gent ligated into pGEM-phtD digested with EcoRV; Apr GentrThis study
    pphtC+A 1.4-kb PCR fragment containing the phtC gene ligated into plasmid pMMB206Δmob; CmrThis study
    pphtD+A 1.5-kb PCR fragment containing the phtD gene ligated into plasmid pMMB206Δmob; CmrThis study
    pJB3395An 800-bp fragment carrying the L. pneumophila strain Philadelphia-1 thyA gene was ligated into the BamHI/SalII sites of pBluescript; Apr27
    pSU19Cloning vector; Cmr53
    pGD257Plasmid pSU18 carrying the E. coli nupC gene; Cmr34
    pSU2719P15A replicon, lacZα, Cmr25
    pKCDBpSU2719::tdk-phtC-phtD-deoBThis study