TABLE 1

Bacterial strains, plasmids, and primers

Strain, plasmid, or primerGenotype, relevant characteristics, or sequenceaSource or reference
Strains
    ATCC 14028sSalmonella enterica serovar Typhimurium ATCC 14028s (WT)ATCC
    KLM001ATCC 14028s fur::bla32
    JV119ATCC 14028s fur::bla/pBAD18-CmThis study
    JV120ATCC 14028s fur::bla/pBAD18-Cm::STM3944This study
    EF434ATCC 14028s/pHR105/pBAD18-KanThis study
    EF435ATCC 14028s/pHR105/pJK714This study
    JV122ATCC 14028s cyaY::FRT-kanThis study
    JK1266ATCC 14028s STM3944::FRTThis study
    JK1264ATCC 14028s STM3944-cyaY::FRT-catThis study
    JV125ATCC 14028s yggX::FRT-catThis study
    JK1206ATCC 14028s cyaY::FRT-kan yggX::FRT-catThis study
    JV129ATCC 14028s iscA::FRT-catThis study
    JV131ATCC 14028s WT/pBAD18-KanThis study
    JV132ATCC 14028s yggX::FRT-cat/pBAD18-KanThis study
    JV133ATCC 14028s yggX::FRT-cat/pBAD18-Kan::yggXThis study
    JV134ATCC 14028s WT/pBAD18-CmThis study
    JV135ATCC 14028s cyaY::FRT-kan/pBAD18-CmThis study
    JV136ATCC 14028s cyaY::FRT-kan/pBAD18-Cm::cyaYThis study
    JK1193ATCC 14028s WT/pRB3This study
    JK1194ATCC 14028s cyaY::FRT-kan/pRB3This study
    JK1196ATCC 14028s yggX::FRT-cat/pRB3This study
    JK1213ATCC 14028s cyaY::FRT-kan/pJK707This study
    JK1216ATCC 14028s yggX::FRT-cat/pJK710This study
    JK1259ATCC 14028s Δedd::FRT-kanThis study
Plasmids
    pKD3bla FRT cat FRT PS1 PS2 ori6K31
    pKD4bla FRT kan FRT PS1 PS2 ori6K31
    pKD46bla araC-ParaB γ β exo oriR101 repA101(Ts)
    pCP20bla cat cI857 λPr flp PSC101 ori(Ts)31
    pBAD18-CmaraC ParaBAD cat33
    pBAD18-Cm::cyaYpBAD18-Cm::cyaYThis study
    pBAD18-Cm::stm3944pBAD18-Cm::stm3944This study
    pBAD18-KanaraC ParaBAD kan33
    pBAD18-Kan::yggXpBAD18-Kan::yggXThis study
    pRB3par RK2 bla stable low-copy-number cloning vector35
    pJK707−750 to −1 ATG stm3944 and cyaY coding region in pRB3This study
    pJK710−361 bp upstream and yggX coding region in pRB3This study
    pHR105cat ParaBAD-feoAB ori/p15A43
    pJK714pBAD18-Kan::stm3944This study
Primers
    cyaYP1ACCTTTTCACCCGCTTGTTGCGTTGCCGCCTGCTCCAGCAGATCCCAGAAGGTTTCGCCGGTGTAGGCTGGAGCTGCTTCThis study
    cyaYP2CAGTGAATTTCATCGCCTGGCTGACGCCCTGTGGCTCACCATTGAAGAACGCCTCGATAGCATATGAATATCCTCCTTAGThis study
    JKP570-cyaY3-P2CGTTGCCGCCTGCTCCAGCAGATCCCAGAAGGTTTCGCCGCATATGAATATCCTCCTTAGThis study
    JKP568-STM3944-P1AAACCGTTTTGCGCACTTGATTAAACATTTGAAAAACGCCGTGTAGGCTGGAGCTGCTTCThis study
    JKP569-STM3944-P2TAAATAGCGCACCGGTGATAAGTATGACGGACGCGTTAACCATATGAATATCCTCCTTAGThis study
    yggXP1GTCGATGAAGAGGATGACTTATGAGCAGAACGATTTTTTGTACTTACCTGCAACGCGACGGTGTAGGCTGGAGCTGCTTCThis study
    yggXP2AGCGTCATTCAGCAACGGGCCTATTTTTTATCTTCCGGCGTATAACCTTCGATATGAACGCATATGAATATCCTCCTTAGThis study
    iscAP1ATGTCGATTACACTTAGCGACAGTGCAGCAGCGCGAGTTAATACCTTCCTGGCCAACCGTGTGTAGGCTGGAGCTGCTTCThis study
    iscAP2ACCACACTCATCTTTCACATTCGGGTTGGAGAATTTAAACCCTTCGTTCAGACCTTCTTTCATATGAATATCCTCCTTAGThis study
    cyaYpBADSacIATGCGAGCTCAGAAGCTGACGCTTCCCCTTThis study
    cyaYpBADSphIrevATGCGCATGCAGCGCTATTCTTCTGGCTCTThis study
    STM3944pBADSacIATGCGAGCTCTACACCAACAATAAAGACATThis study
    STM3944pBADSphIrevATGCGCATGCGCGTCAGCTTCTGGAACCGAThis study
    yggXpBADSacIATGCGAGCTCCGCCCCGGTCGATGAAGAThis study
    yggXpBADSphIrevATGCGCATGCCATAACTCACAGGCCGAATAThis study
    JKP497-cyaYA1AACCGGATCCCCGTCAGGCTGAACAGAGThis study
    JKP499-cyaYBTCCGGTGCCTGCGTTATGATGThis study
    JKP500-cyaYCTAACGCAGGCACCGGAATGAACGACAGTGAATTTCATCGThis study
    JKP501-cyaYDAACCAAGCTTAGCGAAAACTCACCTTTTCACThis study
    JKP505-yggXDAACCAAGCTTCTATTTTTTATCTTCCGGCGTAThis study
    JKP506-yggXFAACCGGATCCTAATCAGGAGATTTTCCTGGCGThis study
    JKP548-edd-P1GAAGCCTATCTTGCCCGCATTGAGCAGGCGAAAACCGCCAGTGTAGGCTGGAGCTGCTTCThis study
    JKP549-edd-P2CCTGCTCCGCACCCGACAGCTTTTCGCGCAGCGCGCCAAACATATGAATATCCTCCTTAGThis study
    JKP82-rpoD_FGTGAAATGGGCACTGTTGAACTGThis study
    JKP83-rpoD_RTTCCAGCAGATAGGTAATGGCTTCThis study
    JKP552-acnA_FTCCTGATGCAGGACTTTACCThis study
    JKP553-acnA_RGGGTTCACTTTCGACGTATCThis study
    JKP554-acnB_FACTCAGACGTTTTCCGTCAGThis study
    JKP555-acnB_RACGGATACCTTTCACACCACThis study
    JKP556-sdaA _FACGTCATTGACTGGGTGAACThis study
    JKP557-sdaA_RGTAAATATCCGGGCTGACTGThis study
    JKP558-sdaB_FCGTCCTTTATCCAGGATGTGThis study
    JKP559-sdaB_RTCTCATGCAGAGACAGGTTGThis study
    JKP560-tdcG_FTTCAAGCTCCCATACCGTAGThis study
    JKP561-tdcG_RAGCGATAGCGATCCGTAAAGThis study
    JKP562-dsdA_FACTCTCGCACGCTATTTCTGThis study
    JKP563-dsdA_RAACAAACAGTGGGTGACTGGThis study
    JKP564-nuoA_FTTGTGTTACTGGCTGGTCTGThis study
    JKP565-nuoA_RGCGTTGACGATTAGCGATACThis study
  • a FRT, FLP recombination target; WT, wild type. All primer sequences are 5′-3′.