TABLE 1

Primers used for cloning

PrimerSequence (5′–3′)a
F- DnaJ-NGGAATTCCATATGAACAATACTGAATTT
R-DnaJ-NCGAGCTCTTCTCCATCAAAGG
F-DnaJ-CCGGCGGCCGCATGAACAATACTGAATT
R-DnaJ-CCCCTCGAGTTATTCTCCATCAAAGGCA
F-ΔA146Ply-NGGAATTCCATATGGCA AAT AAAGCAGTA AAT
R-ΔA146Ply-NCGAGCTCGTCATTTTCTACCTTATCCTCT
F-ΔA146Ply-CCGGCGGCCGC ATGGCAAATAAAGCAGTAAATG
R-ΔA146Ply-CCCCTCGAGTTACTAGTCATTTTCTACCTTATCCTCT
  • a Underlining indicates the recognition sites of restriction enzymes.