TABLE 1

S. aureus strains used in this study

StrainPhenotype, auxotrophismDiagnosisPatient treatment(s)Gene with SNPNucleotide changeAmino acid change
LACWild type, none
221SCVSCV, menadioneAbscessClindamycinmenEG762AL254F
221REVWild typeWild-type menE
377SCVSCV, menadioneBacteremiaVancomycin
PiperacillinmenCG625AA209V
Tazobactam
GentamicinmenFG382AG128E
377COMWild typepsk265menCa (wild type)G625AA209V
G382AG128E
453SCVSCV, menadioneOsteomyelitisVancomycinmenCG514AA172V
Nafcillin
Gentamicin
453COMWild typepsk265menC (wild type)G514AA172V
726SCVSCV, menadioneOsteomyelitisCiprofloxacin, clindamycinmenCG625AA209V
menFG382AG128E
726COMWild typepsk265menC (wild type)G625AA209V
G382AG128E
927SCVSCV, menadioneAbscessClindamycin, trimethoprim-sulfamethoxazolemenFA594GI198M
927REVWild-type menF
  • a psk265 contains the wild-type menC allele from strain LAC (menC accession number SAU300_1735 from the NCBI whole-genome database).