TABLE 1

Quantitative analysis of biofilmsa

BiofilmBiovolume (μm3/μm2)Microcolonies
TotalEPSCellsNo. per total biovolumeSize (×103 μm3)
At 18 h
    S. mutans alone35.6 ± 8.924.3 ± 7.511.5 ± 2.616.3 ± 7.73.6 ± 0.6
    Cospecies66.5 ± 21.5*47.2 ± 18.6*19.3 ± 5.8*33.8 ± 6.0*8.8 ± 5.5*
At 42 h
    S. mutans alone118.2 ± 13.484.3 ± 12.235.5 ± 5.919.4 ± 11.010.7 ± 9.3
    Cospecies251.8 ± 60.9*191.8 ± 60.6*60.2 ± 8.3*12.1 ± 6.830.1 ± 20.5*
  • a Quantitative analysis of biomass (both EPS and total microbial cells) and microcolonies within intact cells was performed using COMSTAT. The data are mean values ± standard deviations (n, ≥15) from at least 3 independent experiments. Asterisks indicate that the values for S. mutans and cospecies biofilms are significantly different from each other (P, <0.01).