TABLE 1

Panels of surface markers used to characterize populations of hematopoietic cellsa

Cell typeMarker detected by:
FITCPEPerCP-Cy5.5PE-Cy7APCAPC-Cy7Pacific Blue
PMNsCD11b+B220CD8CD45+Ly6CCD115Ly6G+
MonocytesCD11b+B220CD8CD45+Ly6C+CD115+Ly6G
B cellsCD11bB220+CD8CD45+Ly6CCD115Ly6G
MacrophagesCD11bCD11chighAutofluor+CD45+I-Ak−CD103Ly6G
CD11b+ DCsCD11b+CD11chighAutofluorCD45+I-Ak+CD103Ly6G
CD103+ DCsCD11bCD11chighAutofluorCD45+I-Ak+CD103+Ly6G
CD4+ T cellsTCRβ+CD3+CD8CD45+CD4+TCRγ/δDx5
CD8+ T cellsTCRβ+CD3+CD8+CD45+CD4TCRγ/δDx5
γ/δ T cellsTCRβCD3+CD8CD45+CD4TCRγ/δ+Dx5
NK cellsCD3IFN-γ+/−CD45+NK1.1+
ProgenitorsCD31+Ly6Cmid
Peritoneal monocytesB220F4/80CD115+
Peritoneal macrophagesB220F4/80+CD115+
  • a See reference 23. Dead cells were excluded from the analysis. Positive markers are in boldface type. CD45 was not included for cells isolated from bone marrow (progenitors) or from peritoneal wash specimens (monocytes and macrophages), as virtually all cells isolated from these sites are of hematopoietic origin. Abbreviations: PMNs, polymorphonuclear cells (neutrophils); DCs, dendritic cells; Autofluor, autofluorescence.