C. trachomatis isolates and transformants used in this study

Strain nomenclatureDescription
L2 P 25667RNaturally occurring plasmid-free isolate of C. trachomatis serovar L2 from a patient with proctocolitis (22); the genome has been sequenced and deposited at GenBank (reference sequence NC_020930) (23); for brevity, we use the designation L2P here
L2 P.pGFP::SW2L2P 25667R transformed with a recombinant chlamydial plasmid (pGFP::SW2) derived from Swedish new variant C. trachomatis serovar E (14); this plasmid (pGFP::SW2) possesses an Escherichia coli origin of replication, bla, cat, and a red-shifted green fluorescent protein gene; the latter two are under the control of a Neisseria meningitidis promoter
L2 P.pCDS5KOL2P 25667R transformed with pGFP::SW2 with a functional deletion in plasmid CDS5 which encodes the putative virulence effector pgp3; the creation of this recombinant plasmid was described in detail previously (15)
L2 434/BuCommonly referenced laboratory strain of C. trachomatis serovar L2 cultured from a bubo of a patient with LGV (25); the genome of this isolate has been sequenced and published elsewhere (26) (GenBank reference sequence NC_010287); in the present study, this isolate was used only as a comparator for L2P.pGFP::SW2