TABLE 1

Strains and plasmids used in this study

Strains and plasmidsGenotype and/or commentsSource or reference
S. marcescens
    Wild typeS. marcescens RM66262; clinical isolate17
    rcsBrcsB::pKNOCK-Gmr16
    rcsArcsA::pKNOCK- Gmr16
    rcsCrcsC::pKNOCK-Gmr16
    rcsFrcsF::pKNOCK-Gmr16
    fliAfliA::pKNOCK-CmrThis work
    flhDflhD::pKNOCK-Cmr4
    shlBshlB::pKNOCK-SprThis work
    wecGwecG::pKNOCK-Cmr16
    phlAΔphlAB::Cmr17
    wecG rcsBwecG::pKNOCK-Cm; mini-Tn5::rcsB; Kmr16
    phlA rcsBΔphlAB::Cm; rcsB::pKNOCK-GmrThis work
    phlA shlBΔphlAB::Cm; shlB::pKNOCK-SprThis work
    fliA/pBB2fliA::pKNOCK-Cmr/pBB2; KmrThis work
    flhD/pBB2flhD::pKNOCK-Cmr/pBB2; KmrThis work
    fliA/pBB2::fliAfliA::pKNOCK-Cmr/pBB2::fliA; KmrThis work
    flhD/pBB2::flhDCflhD::pKNOCK-Cmr/pBB2::flhDC; Kmr4
    fliA/pBB2::flhDCfliA::pKNOCK-Cmr/pBB2::flhDC; KmrThis work
    flhD/pBB2::fliAflhD::pKNOCK-Cmr/pBB2::fliA; KmrThis work
    wt/pBB2Wild-type strain/pBB2; KmrThis work
    wt/pBB2::fliAWild-type strain/pBB2::fliA; KmrThis work
    wt/pBB2::flhDCWild-type strain/pBB2::flhDC; KmrThis work
    shlB/pBB2shlB::pKNOCK-Spr/pBB2; KmrThis work
    shlB/pBB2::fliAshlB::pKNOCK-Spr/pBB2::fliA; KmrThis work
    shlB/pBB2::flhDCshlB::pKNOCK-Spr/pBB2::flhDC; KmrThis work
    rcsB/pBB1rcsB::pKNOCK-Gmr/pBB1 lacI; CmrThis work
    rcsB/pBB1::rcsBrcsB::pKNOCK-Gmr/pBB1 lacI::rcsB; CmrThis work
    fliA/pBB5fliA::pKNOCK-Cmr/pBB5; GmrThis work
    flhD/pBB5flhD::pKNOCK-Cmr/pBB5; GmrThis work
    fliA/pBB5::rcsBfliA::pKNOCK-Cmr/pBB5::rcsB; GmrThis work
    flhD/pBB5::rcsBflhD::pKNOCK-Cmr/pBB5::rcsB; GmrThis work
    wecG/pBB2wecG::pKNOCK-Cmr/pBB2; KmrThis work
    wecG/pBB2::fliAwecG::pKNOCK-Cmr/pBB2::fliA; KmrThis work
    wecG/pBB2::flhDCwecG::pKNOCK-Cmr/pBB2::flhDC; KmrThis work
    wt/ppromshlBA-gfpWild-type/pPROBE(NT)::promshlBA; KmrThis work
    fliA/ppromshlBA-gfpfliA::pKNOCKCmr/pPROBE(NT)::promshlBA; KmrThis work
    flhD/ppromshlBA-gfpflhD::pKNOCK-Cmr/pPROBE(NT)::promshlBA; KmrThis work
    wecG/ppromshlBA-gfpwecG::pKNOCK-Cmr/pPROBE(NT)::promshlBA; KmrThis work
    rcsB/ppromshlBA-gfprcsB::pKNOCK-Gmr/pPROBE(NT)::promshlBA; KmrThis work
E. coli
    XL1-BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lacTcRStratagene
    W3110λ IN(rrnD-rrnE)1 rph-150
    W3110/pT7T7 overexpression vector; AmprThis work
    W3110/pES14pT7–5::shlBA; AmprThis work
    W3110/pBB2pBB2; KmrThis work
    W3110/pphlABpBB2::phlAB; KmrThis work
    BL21(DE3), pLysSF ompT hsdSB(rB mB) gal dcm (DE3) pLysS(Cmr)Stratagene
Plasmids
    pBB1pBBR1-MCS1 Cmr; broad host range51
    pBB2pBBR1-MCS2 Kmr; broad host range51
    pBB5pBBR1-MCS5 Gmr; broad host range51
    pflhDCpBBR1-MCS2::flhDC; Kmr4
    pT7T7 overexpression vector; Ampr52
    pES14pT7-5:shlBA; Ampr7
    pphlABpBBR1-MCS2::phlAB; Kmr17
    pBB2::shlBApBBR1-MCS2::shlBA; KmrThis work
    pBB1::rcsBpBBR1-MCS1::rcsB; CmrThis work
    pBB5::rcsBpBBR1-MCS5::rcsB; GmrThis work
    pBB2::fliApBBR1-MCS2::fliA; KmrThis work
    pBB2::flhDCpBBR1-MCS2::flhDC; Kmr4
    ppromshlBA-gfppPROBE(NT)::promshlBA; KmrThis work