TABLE 2

Primers used in this study

PrimerSequence (5′–3′)a
PromShlBFwEcoRICGGAATTCTATTTCTCGCCGCTGG
PromShlBRvBamHICGGGATCCGTGCTGACCAACAGC
FwmutShlB (BamHI)CGCGGATCCCGCGTGCAAATTGTTC
RevmutShlB (XhoI)CCGCTCGAGCCGTACGGCAGCGAAT
PromflhDFwAAATGGCGGTCAATAAGTGAG
PromflhDRvGTACCCATATTCCCCATC
FliAserratiaNterTCGCCCGGGGTGAGCGATCTGTATAC
FliActerrevserratiaTCCAAGCTTCAGGTATCGTTCGCC
FlhDserratiaNterTCGCCCGGGATGGGGAATATGGGTAC
FlhDcterrevserratiaTCCAAGCTTCAGGCTCTTTTCTTCG
RcsBFwXhoICCGCTCGAGATGAATAACCTGAACG
RcsBRvBamHICGGGATCCTTAGTCTTTGTCCAACGG
rcsBHis-ParallelNcoIFwCATGCCATGGGAATGAATAACCTGAACG
rcsBHis-Parallel XhoIRvCCGCTCGAGTTAGTCTTTGTCCAACGG
  • a Restriction sites are underlined.