TABLE 1

SV-AUC results for DB fusion peak species in the presence of LDAO or OPEO

Detergent present and peak speciesSedimentation coefficient (Svedberg units)aRelative % of massb
With LDAO
    Fragment3.19 ± 0.003715 ± 0.39
    Monomer4.82 ± 0.001654 ± 0.53
    Dimer7.03 ± 0.004326 ± 0.82
    Trimer9.60 ± 0.0176 ± 0.75
With OPOE
    Monomer5.01 ± 0.007216 ± 0.66
    Dimer7.64 ± 0.00409 ± 0.072
    Trimer and tetramer11.15 ± 0.01045 ± 1.39
    Hexamer15.29 ± 0.00505 ± 1.51
    Octamer17.67 ± 0.0183 ± 0.90
    Higher-order aggregates22.43 ± 0.0216 ± 1.81
25.19 ± 0.01711 ± 3.31
34.84 ± 0.0412 ± 0.60
41.23 ± 0.0481 ± 0.30
  • a Values are means ± standard errors; errors for sedimentation coefficients were determined using Monte-Carlo simulation with a 95% confidence interval.

  • b The relative percentages of mass that could be attributed to each DB fusion species are expressed as means ± standard errors; errors were determined based on the root mean square deviation of the fit.