TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or characteristicsaReference or source
E. coli strains
    EDL933(Δstx1-2)O157:H7 Δstx1 Δstx224
    EDL933(Δstx1-2, Δ2)O157:H7 Δstx1 Δstx2 ΔpatE ΔpsrA24
    EDL933(Δstx1-2, Δ3)O157:H7 Δstx1 Δstx2 ΔpatE ΔpsrA ΔpsrB24
    BL21(DE3)F ompT hsdSB(rB mB) gal dcmNew England BioLabs
    TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(Strr) endA1 λInvitrogen
Plasmids
    pCR2.1-TOPOHigh-copy-number vector; Apr KanrInvitrogen
    pGEM-T EasyHigh-copy-number vector; AprPromega
    pACYC184Low-copy-number vector; Cmr TcrNew England BioLabs
    pMAL-c2xExpression vector for N-terminal MBP-fusion proteinsNew England BioLabs
    pJY1pACYC184 plus psrAThis study
    pJY2pACYC184 plus psrBThis study
    pJB23pGEM-T Easy plus hdeA-prom fragment24
    pJB29pPJ1433 plus hdeA-lacZ-1 construct24
    pJB37pGEM-T Easy plus hdeA-control fragment24
    pJB50pPJ1433 plus hdeA-lacZ-4 construct24
    pJB51pPJ1433 plus hdeA-lacZ-3 construct24
    pJB52pPJ1433 plus hdeA-lacZ-2 construct24
    pJP1433Single-copy-number transcriptional fusion vector; Tpr24
    pTR1pGEM-T Easy plus psrBThis study
    pTR2pMAL-c2x plus psrBThis study
  • a Apr, ampicillin resistance; Kanr, kanamycin resistance; Strr, streptomycin resistance; Tpr, trimethoprim resistance.