Transmission of ΔmotB mutant spirochetes from freshly infected nymphs to mice

B. burgdorferi clone used to infect nymphsaNo. of mice infected/total no. of mice tested
  • a Ten nymphs immersed in WT or motBcom mutant spirochetes and 30 nymphs immersed in ΔmotB mutant spirochetes were allowed to feed per C3H/HeN mouse using the capsule method. Immediately after most ticks dropped off, mice were sacrificed. Two sections of skin tissue from the tick bite site were assessed for the presence of B. burgdorferi by regrowth in culture medium and detection of B. burgdorferi genomes using flaB-enolase gene-specific PCR.