TABLE 1

Strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeaReference
Strains
    CFT073Wild-type parent strain (ATCC 700928)15
    CFT073 Δfurfur mutant of CFT073This work
    CFT073 ΔryhBryhB mutant of CFT073This work
    CFT073 Δlrplrp mutant of CFT073This work
    CFT073 Δfur Δlrpfur lrp mutant of CFT073This work
    CFT073 Δfur ΔryhBfur ryhB mutant of CFT073This work
Plasmids
    pKO3Temperature-sensitive vector for gene targeting; sacB; Cmr17
    pTrc99AVector for IPTG-inducible expression; Apr18
    pTrc99AfurFur overexpression plasmid; AprThis work
    pTrc99Afur6HisC-terminal Fur-His6 overexpression plasmid; AprThis work
    pTrc99AryhBRyhB overexpression plasmid; AprThis work
    pUC19Very-high-copy-number plasmid; Apr19
    pUC19ryhBRyhB overexpression plasmid; AprThis work
    pTurboGFP-BGFP expression plasmid; AprEvrogen
  • a Cmr, chloramphenicol resistance; Apr, ampicillin resistance.