TABLE 2

Oligonucleotide primers used in this study

PrimerSequencePurpose
JG2093 JMM5′-CTTCACGTTCTTCCGTAAGGTCTC-3′Forward yihO
JG2094 JMM5′-GTTTTCTACATAAGCGCCAGCA-3′Reverse yihO
JG26745′-CCAATATTACCCAGTACGGTACGCAGAAAACAGC-3′Forward csgA
JG26755′-GGTTCGTTTAATGTGACCTGAGGGATCACCG-3′Reverse csgA
JG26765′-GCTTGTTTTCGCCAGCATGAATTACTCTGC-3′Forward wcaM
JG26775′-CGTGGTATCTACCGTGCATAGCGGTATTCC-3′Reverse wcaM
JG26825′-GGCAGCATTACGCCCCGTCGTCAATACGGG-3′Forward bcsE
JG26835′-CGGCGCAGAGTTCCGGCTGGTTTTACATAACG-3′Reverse bcsE
JG27005′-AACTGATAAACAGTTAAAATAGTGACATGAGTGTAGGCTGGAGCTGCTTC-3′λ-Red deletion of forward bcsE
JG27015′-CGATATCGCTGATGGTCATCATGATGCACGCATATGAATATCCTCCTTAG-3′λ-Red deletion of reverse bcsE