TABLE 1

Mitoxosome gene expression of macrophages measured by qRT-PCRa

Gene IDDescriptionGenBank accession no.LPS/IFN-γIL-4SYLX10
Fold changeP valueFold changeP valueFold changeP value
atp5a1ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscleNM_001001937−1.833ND−10.0170.385−1.6800.410
atp5c1ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1NM_00100197319.2020.117−2.2370.3438.9800.000
atp5oATP synthase, H+ transporting, mitochondrial F1 complex, O subunitNM_001697−8.5940.157−1.5130.45511.0220.000
bpolPolymerase (DNA directed), betaNM_002690−4.4860.045−1.6260.2036.1320.001
cenpbCentromere protein BNM_0018102.0420.4476.5630.581−26.2330.119
cox5bCytochrome c oxidase subunit VbNM_001862−4.2930.002NC0.0235.0920.000
cox7a2Cytochrome c oxidase subunit VIIa polypeptide 2 (liver)NM_001865−5.7420.077NC0.6238.2190.001
cyb5r3Cytochrome b5 reductase 3NM_0003981.8760.7842.4960.906−1.6220.220
cycn1Cyclin 1NM_006835−2.4570.2911.8990.832NC0.429
drp1Dynamin 1-likeNM_012063−2.6720.224NC0.9551.9120.190
fhl1Four and a half LIM domains 1NM_001449−6.3890.230NC0.488−1.9350.185
fis1Fission 1 (mitochondrial outer membrane)NM_016068−4.7500.000−1.5200.0475.1420.000
ho1Heme oxygenase 1GQ221778.11.5100.831NC0.763−1.6170.252
hspc051Ubiquinol-cytochrome c reductase, complex III subunit XNM_013387−5.5930.002NC0.1375.5440.006
lsp1Lymphocyte-specific protein 1NM_002339−1.8560.339NC0.478−3.0820.170
mfn1Mitofusin 1NM_0335401.5360.664NC0.7381.8650.255
mfn2Mitofusin 2NM_014874NC0.3601.6810.8352.8740.436
mt-16SMitochondrially encoded 16S RNAGQ369957.1
mt-atp6Mitochondrially encoded ATP synthase 6GQ369957.1−3.1760.033NC0.6475.7270.007
mt-atp8Mitochondrially encoded ATP synthase 8GQ369957.1−3.4640.080NC0.8812.0680.189
mt-co1Mitochondrially encoded cytochrome c oxidase IGQ369957.1−1.8930.310NC0.6132.3770.098
mt-co2Mitochondrial cytochrome c oxidase subunit IIGQ369957.1−1.7580.318NC0.9812.2420.110
mt-co3Mitochondrially encoded cytochrome c oxidase IIIGQ369957.1−3.3870.002NC0.3078.8190.006
mt-cytbMitochondrially encoded cytochrome bGQ369957.1−2.9570.108NC0.4384.3260.010
mt-nd1Mitochondrially encoded NADH dehydrogenase 1GQ369957.1−4.9700.000−1.5960.0176.8230.012
mt-nd2Mitochondrially encoded NADH dehydrogenase 2GQ369957.1−2.9450.150NC0.5464.2410.011
mt-nd3Mitochondrially encoded NADH dehydrogenase 3GQ369957.1−3.0330.026NC0.3815.6730.011
mt-nd4NADH dehydrogenase 4GQ369957.1−1.9620.308NC0.9902.7110.091
mt-nd4lMitochondrially encoded NADH dehydrogenase 4LGQ369957.1−2.3400.234NC0.9292.9230.026
mt-nd5Mitochondrially encoded NADH dehydrogenase 5GQ369957.1−1.5560.3701.6540.985NC0.704
mt-nd6NADH dehydrogenase subunit 6GQ369957.1−4.3730.032NC0.4424.1310.006
ndufa5NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 5NM_005000−7.3250.048NC0.3293.8930.017
ndufb5NADH dehydrogenase (ubiquinone) 1 beta subcomplex 5NM_002492−5.2770.003NC0.4555.1910.004
nrf1aNuclear respiratory factor 1NM_001040110−1.8250.342NC0.961NC0.347
nrf2Nuclear factor, erythroid 2-like 2NM_0061641.5660.913NC0.527NC0.401
opa1Optic atrophy 1NM_015560−2.6780.169NC0.9692.5290.012
parkinParkin RBR E3 ubiquitin protein ligaseNM_004562−2.3440.290NC0.607−1.7170.218
ppargc1aPeroxisome proliferator-activated receptor gamma, coactivator 1 alphaNM_013261−3.5400.503NC0.9782.9210.180
pink1PTEN-induced putative kinase 1NM_032409−5.8090.128−1.5140.3902.567ND
polg1Polymerase (DNA directed), gammaNM_0026933.4060.5756.2860.901−15.2560.126
rps2Ribosomal protein S2NM_002952NC0.4262.6750.646NC0.346
sdhdSuccinate dehydrogenase complex, subunit D, integral membrane proteinNM_003002−1.6040.299NC0.4444.1320.002
sod2Superoxide dismutase 2, mitochondrialNM_0006364.0540.017−5.3950.1691.8760.249
tfamMitochondrial transcription factor ANM_003201−8.6590.001NC0.6805.306ND
uqcrbUbiquinol-cytochrome c reductase binding proteinNM_006294−6.8590.004−1.8430.0197.5020.001
uqcrc1Ubiquinol-cytochrome c reductase core protein INM_003365−3.1620.1981.8430.3612.0940.174
  • a A panel of 45 mitoxosome genes were used for the evaluation of THP-1 macrophages treated with IFN-γ/LPS, IL-4, and SYL as described in Materials and Methods. Fold changes in gene expression compared to no-treatment controls (N) were calculated by using the 2−ΔΔCT method. Fold change values > |1.5| are shown. Upregulation data are highlighted with underlining, downregulation data are highlighted in bold, and P values < 0.05 are highlighted in italics. ND, not detectable or not determined; NC, no change or fold change < |1.5|. ID, identifier.