TABLE 2

Doubling times of pe-ppe deletion mutants in Pi-rich 7H9 mediuma

pe-ppe deletionDoubling timeb (h), WT backgroundP value (vs WT)Doubling timeb (h), ΔpstA1 backgroundP value (vs ΔpstA1)
None19.41 ± 1.5919.71 ± 1.70
Δpe32-ppe6520.99 ± 0.500.131418.92 ± 0.920.4704
Δppe25-pe1927.63 ± 4.290.000427.73 ± 2.19<0.0001
Δppe25-ppe2620.87 ± 0.340.156819.50 ± 0.880.8509
Δppe27-pe1928.32 ± 2.55<0.000125.86 ± 2.190.0005
Δppe27-pe19 pMVppe27-pe1923.25 ± 0.980.023621.77 ± 1.130.1554
Δppe2718.54 ± 0.240.401818.15 ± 0.330.1937
Δpe1922.67 ± 0.870.00322.95 ± 2.520.0137
Δpe19 pMV-pe1920.70 ± 0.930.221019.65 ± 0.610.9581
  • a Doubling times were determined from optical density measurements taken during the first 72 h of growth after dilution to an OD600 of 0.05 in 7H9 medium.

  • b Means ± standard deviations of doubling times from three independent cultures.