TABLE 1

MG192 variant sequences of M. genitalium isolates from two chimpanzees infected with strain G37T

Animal no. and no. of wk p.i.No. of unique sequences/total no. of plasmid or MSCC clones analyzedaAvg no. ± SD (%) of:
Recombination events relative to G37T MgParsNucleotide changes relative to G37TbDeduced amino acid changes relative to G37Tb
Animal no. A52
    01/5000
    54/80.75 ± 0.531.5 ± 34.4 (2.2)12.8 ± 9.6 (2.7)
    108/105.6 ± 0.7130.8 ± 17.5 (9.2)46.1 ± 5.2 (9.7)
    133/83 ± 0111.7 ± 5.8 (7.8)34 ± 2.6 (7.2)
    Spearman's testc0.519 (P < 0.040)0.573 (P < 0.020)0.502 (P < 0.047)
Animal no. 1125
    01/10000
    58/152.3 ± 1.338.9 ± 20.5 (2.7)14.5 ± 7.5 (3.1)
    91/153130 (9.1)48 (10.1)
    114/29d7.6 ± 1.5117.9 ± 13.3 (8.3)40 ± 7.3 (8.4)
    Spearman's testc0.883 (P < 0.0001)0.841 (P < 0.0001)0.841 (P < 0.0001)
  • a All clones are plasmid clones except for the 29 clones at 11 weeks p.i. from animal no. 1125 as explained below in footnote d.

  • b Compared to the published G37T MG192 variable region (1,423 nucleotides or 474 amino acids). Changes include base substitutions, deletions, and insertions in regions flanking the AGT triplet repeat region. Values are averages ± standard deviations for more than one variant.

  • c Spearman's rank correlation coefficient.

  • d Including variants 11j, 11k, 11l, and 11m from 17 MSCC clones and 12 plasmid clones derived from other three MSCC clones. The 12 plasmid clones are included here, since four variants identified from them were also found in the uncloned swab isolate. From the same three MSCC clones above, we obtained an additional seven plasmid clones, which contained three unique sequences (variants 11n, 11o, and 11p); they are not included in this table because they were not detected in the uncloned swab isolate and might have been introduced in vitro during the MSCC process. See Table 2 for more details.