TABLE 2

MG192 variant sequences identified in 20 M. genitalium MSCC clones from the 11-week swab isolate from chimpanzee no. 1125

No. of MSCC clones (clone designation)MG192 variant by direct sequencingNo. of plasmid clonesMG192 variant from plasmid clones
611jaNAcNA
1111kaNANA
1 (K4)Mixture411ja
211 ma
1 (K13)Mixture211ja
211ka
111la
1 (K20)Mixture111ja
511nb
111ob
111pb
  • a Also found in the uncloned swab isolate by sequencing of cloned PCR products or variant-specific PCR.

  • b Not detected in the uncloned swab isolate by variant-specific PCR and thus excluded for analysis shown in Table 1 and Fig. 1.

  • c NA, not cloned into plasmid since direct sequencing showed clear, homogeneous sequences.