Mouse infectivity of B. burgdorferi clonal isolates of cyaB and PEP-PTS mutants

Strain or cloneMutated geneInsertion sitebInsertion ratiocNo. of positive cultures/totala% positive sitesAdditional plasmid(s) absentd
Gene no.DesignationEarSkinJointHeartBladderAll sites
5A18NP1 (WT)3/33/33/33/33/315/15100
T11TC373BB0723cyaB7621430.023/33/33/33/33/315/15100lp5, lp38
T10TC291BB0645ptsG6841110.450/30/30/30/30/30/150lp5, lp28-2
T09TC107BB0645ptsG6846340.780/30/30/30/30/30/150lp5, lp38
T11P02A09e,fBB0629fruA26611520.200/30/30/30/30/30/150cp9, lp5
T05TC436e,fBB0629fruA26602100.703/33/33/33/33/315/15100cp9, lp5
T06TC053e,fBB0629fruA26601920.713/33/33/33/33/315/15100cp9, lp5
T08P02E04BB0116malX11133410.523/33/33/32/31/312/1580lp5, lp28-2
T05TC295BBB05chbAcp26, 42290.423/33/33/33/33/315/15100cp9, lp5
T08P01C04BBB04chbCcp26, 35010.233/33/33/33/33/315/15100lp5, lp21
T09TC250BB0630fruK6618010.213/33/33/33/33/315/15100cp9, lp5
  • a Groups of 3 C3H/HeN mice were each inoculated subcutaneously with 5 × 105 organisms of the strains shown. The mice were euthanized on day 28 postinoculation, and cultures were inoculated with the indicated aseptically collected tissues. Cultures were monitored for presence of B. burgdorferi by dark-field microscopy as described in Materials and Methods.

  • b Transposon insertion site in the chromosome sequence (GenBank accession no. AE000783.1) or cp26 (GenBank accession no. AE000792.1) for chbA and chbC. 5A18NP1 is the parental strain.

  • c Number of nucleotides from the 5′ end of the gene to the insertion site divided by the total number of nucleotides in the gene.

  • d 5A18NP1 and all transposon mutant progeny lack lp28-4 and lp56. Plasmids absent in 5A18NP1 and the mutants are not required for mouse infectivity (60).

  • e The initial culture of the clone contained a second transposon mutant, as determined by PCR (see Materials and Methods). Each clone was separated from the coisolate by replating, and its purity was confirmed by a second PCR.

  • f One fruA2 mutant clone (T11P02A09) was noninfectious, whereas the remaining three were infectious.