TABLE 3

Roles of ptsG and cyaB in tick colonization and transmission of B. burgdorferi from ticks to mice

Strain (mutated gene)Insertion ratioUnfed ticksFed ticksMouse infection by tick transmission
No. of positive cultures/totalMean no. of spirochetes per field ± SDNo. of positive cultures/totalMean no. of spirochetes per field ± SDNo. of positive cultures/total (no. of positive mice/total)No. of mice with positive serology/total
5A18NP17/100.05 ± 0.0615/154.5 ± 4.112/12 (3/3)3/3
T11TC373 (cyaB)0.0210/100.14 ± 0.1517/173.3 ± 1.712/12 (3/3)3/3
T08TC498 (cyaB)0.937/100.04 ± 0.0420/221.2 ± 0.912/12 (3/3)3/3
T10TC291 (ptsG)0.4510/100.13 ± 0.1222/223.9 ± 3.80/12 (0/3)0/3
ptsG::pKFSS1-ptsG9/100.08 ± 0.0723/236.3 ± 6.74/12 (1/3)1/3