TABLE 6

Validation of differential gene expression of selected genes in the ptsG mutant using qRT-PCR

Gene no.Gene nameFold difference (ptsG/WT) determined by:
RNA-seqqRT-PCRa
BB_F0041vlsE17.34.4
BB_0844Hypothetical gene13.418.6
BB_A24dbpA4.46.1
BB_B25dbpB5.01.8
BB_B19ospC4.01.1
BB_B04chbC−2.21.5
BB_0408fruA11.35.3
BB_0407manA1.3−1.04
BB_A74osm282.21.9
BB_0733plzA1.61.7
BB_0771rpoS2.11.7
BB_0450rpoN1.2−1.5
BB_0448hpr1.2−1.6
  • a Results for the fold difference determined by qRT-PCR analysis are representative of one experiment performed on 3 or 4 technical replicates of the same RNA samples used for RNA-seq.