TABLE 2

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant descriptionSource or reference
Strains
    E. coli
        K-12 (MC4100)62
        JW5267(BW25113) ΔslyA::Kn28
    S. flexneri
        2457TS. flexneri serotype 2a63
        AWY3(2457T) virB::Tn536
        BS1032457T cured of the virulence plasmid49
        BS184(2457T) mxiC-lacZ31
        NWG1(2457T) ΔslyA::KnThis study
        NWG11(2457T) ΔslyA::lacZThis study
        NWG13(2457T) ΔphoP::KnsThis study
        NWG14(2457T) ΔslyA::lacZ hns::Tn10This study
        NWG15(2457T) ΔslyA::lacZ ΔphoP::KnsThis study
Plasmids
    pBAD18Arabinose inducible, pBRori; Ampr64
    pATM324pBAD18-virB; Ampr65
    pNWG03pBAD18-his-slyA; AmprThis study
    pAFW04pACYC184-PicsP-lacZ; Cmr19
    pSRG58pACYC184-PphoP-phoPQ; Spcr33
    pNWG07pACYC184; SpcrThis study
    pBR322pMB1ori; Ampr66
    pNWG13pBR322-PslyA-slyA; AmprThis study