TABLE 3

Identification of linocin and OmpW from 2D blots probed with sera from Bcc-colonized CF patients compared with control blots using sera from CF patients with no history of Bcc infection

GI sequence identifierProtein (species)Molecular mass (kDa)/pIaStrainSequence coverage (%)Scoreb
GI 161524680OmpW family protein22.9/9BC727–8554–142
C13941074–78
LMG1030329–85108–253
C196236–8443–222
GI 221198593Linocin (B. multivorans)28.8/4.8BC773.8–78.2155–572
GI 170735569Linocin (B. cenocepacia)c28.8/4.8C139441.7–50.976–89
GI 221198593Linocin (B. multivorans)28.8/4.8LMG1301034–81193–488
GI 221198593Linocin (B. multivorans)28.8/4.8C196248.3–90105–141
  • a Theoretical molecular masses (kilodaltons) and isoelectric points were determined by Mascot analysis.

  • b Ranges represent MS/MS ion scores determined by mass fingerprinting. Only scores that were deemed to be significant by Mascot analysis (P < 0.05) are reported.

  • c Query coverage of 96%; 92% identity.