TABLE 1

Primer sequences for qRT-PCRa

PrimerSequenceProduct size (bp)NCBI accession no.
IL-32f5′-TCAAGAGAACAGTCCCGAAACC-3′71Consensus sequence
IL-32r5′-AGCGTACTTCTTGCTGTGCTTC-3′
IL-8f5′-TGGGCCACACTGTGAAAAT-3′92NM_173925.2
bIL-8r5′-TCATGGATCTTGCTTCTCAGC-3′
IL-6f5′-TGCTGGTCTTCTGGAGTATC-3′153EU276071
IL-6r5′- GTGGCTGGAGTGGTTATTAG-3′
RPL19f5′-TACTGCCAATGCTCGAATGC-3′114NM_001040516.1
RPL19r5′-TGATACATGTGGCGGTCAATC-3′
PPIAf5′-ATGGCAAGACCAGCAAGAAG-3′201XM_002690515.2
PPIAr5′-CTTGGAGGGGGATAAGGAAA-3′
  • a Primer sequences were designed using primer 3. The RPL19 and PPIA genes were used as housekeeping genes. All melting temperatures were 60°C. The relative quantification of the mRNA levels of the target genes was determined using CFX Manager based on the threshold cycle method.