TABLE 1

Protein spots that were differentially expressed in epimastigote stage of TcI isolates of T. cruzia

Protein spotProtein name (gene)NCBI accession no.Mol mass (Da)Peptide countProtein scoreProtein score (CI%)pIE value
Spots conserved in the three isolates
    1Chain A, tyrosine aminotransferase (TcTAT)gi|613741846,120.3121881005.723.155E–13
    3Tyrosine aminotransferase (TcTAT)gi|7140731146,238.5134421005.821.256E–38
    4Tyrosine aminotransferase N-term (TcTAT)gi|7165533927,698.9124001005.711.991E–34
    5l-Tyrosine:2-oxoglutarate aminotransferase (TcTAT)gi|116860646,137.4207371005.823.972E–68
    6Tyrosine aminotransferase (TcTAT)gi|7141393846,100.4165371005.663.972E–48
    7NADPH flavin oxidoreductase (old yellow enzyme, TcOYE)gi|6174194242,191.6199021006.031.256E–84
    8NADPH flavin oxidoreductase (old yellow enzyme, TcOYE)gi|6174195042,233.6188891005.972.506E–83
    9l-Threonine 3-dehydrogenase (TcTDH)gi|7140616036,880.781811006.711.581E–12
    10NADPH flavin oxidoreductase (old yellow enzyme, TcOYE)gi|6174195242,080.5207491006.032.506E–69
    11Aldo-keto reductase/quinone oxido-reductase (AKR)gi|18939613532,485.5134991007.181.256E–43
    12Aldo-keto reductase/quinone oxido-reductase (AKR)gi|1427917431,764.2133761008.582.506E–31
Protein spots differentially expressed in COL (compared to TCC)
    13Proline racemase B (TcPRACB)gi|7481222038,440.6124421005.441.256E–38
    14Proline racemase N-term (TcPRACB)gi|7140079432,411.5114351005.636.295E–38
    15Major paraflagellar rod protein 2 (TcPAR2)gi|16217969,504.9193551005.856.295E–30
    16Paraflagellar rod protein N-term (TcPAR)gi|7141138742,471.9163551006.126.295E–30
    17Paraxonemal (paraflagellar) rod protein (TcPAR2)gi|7139880131,336.1101641005.957.924E–11
    182-Hydroxyacid dehydrogenase N-term, d-isomer (2-Hacid-dh)gi|7140618728,516.7165671006.173.972E–51
    20Short interspersed repetitive element (TcSIRE)gi|191117211,565105301005.081.991E–47
    212-Hydroxyacid dehydrogenase, d-isomer (2-Hacid-dh)gi|7140618922,228.2112961005.875E–24
    23HpcH/HpaI aldolase (TcP28)gi|455845629,886.271561006.065E–10
    24Pyridoxal kinase (TcPDXK)gi|7141857933,273.641251005.936.295E–07
    25NADP-dependent malic enzyme N term (TcME1)gi|7166250662,705.5123071006.553.972E–25
    26NADP-dependent malic enzyme N term (TcME1)gi|7165146562,634.4143001006.371.991E–24
    27Cytochrome c oxidase subunit IV (TcCOXIV)gi|7166041938,924.4163511005.721.581E–29
    28Tryparedoxin peroxidase (TcTXNPx)gi|1722495322,246.1114131005.969.976E–36
    29Tryparedoxin peroxidase (TcTXNPx)gi|7139951422,375.1114111006.321.581E–35
    30Tryparedoxin peroxidase (TcTXNPx)gi|438865525,545.3221671007.562.506E–28
    31Calpain-like cysteine peptidase (TcCAPN1)gi|7141100612,798.361791005.071.256E–11
    32Beta tubulin of T. cyclops (TcTUBB)gi|9198319744,496.8134101005.681.991E–35
    33Beta tubulin of T. danilewskyi (TdTUBB)gi|7422992649,695.8123911004.741.581E–33
    34Alpha tubulin of T. cyclops (TcTUBA)gi|9198319649,777.5196771004.931.991E–61
    35Alpha tubulin of T. brucei (TbTUBA)gi|11550428349,755.5216461004.932.506E–58
    36Elongation factor 1α (TcEF1A)gi|7140891049,083.4194161008.935E–36
    37Ribokinase (TcRBKS)gi|7165974335,262.671581006.953.155E–10
    38Heat shock protein 70 partial (TcHSP70)gi|1062674,233.7316261005.642.506E–56
    39Heat shock protein HSP70 (TcHSP70)gi|16211771,210.1306231005.425E–56
    40Heat shock 70-kDa protein, mitochondrial (TcHSP70-10)gi|7140751570,946245161005.752.506E–45
Spots differentially expressed in SYL (compared to TCC)
    53Cytochrome c oxidase subunit IV (TcCOXIV)gi|7166785438,884.4153391005.722.506E–28
    54Tryparedoxin peroxidase (TcTXNPx)gi|7141320722,376.1103571005.693.972E–30
    55Tryparedoxin peroxidase (TcTXNPx)gi|438865525,506.161341007.627.924E–08
    56Elongation factor alpha G5 (TcEFA-G5)gi|5242404649,067.4184051008.936.295E–35
    57Heat shock protein 70 (TcHSP70)gi|5065975671,329.9305241005.063.972E–46
    58Glucose-regulated protein 78 (TcHSPA5 or TcGRP78)gi|7141550571,271.9295101005.099.976E–45
    59Glucose-regulated protein 78 (TcHSPA5 or TcGRP78)gi|34983871,488253651005.073.155E–30
75- to 77-kDa antigen (TcHSP70)gi|109775041,716.5172731004.995E–21
    60Mitochondrial heat shock protein (TcHSP70mt)gi|20527887070,969.1255271005.871.99E–46
    61Heat shock protein 70 (TcHSP70)gi|20527886871,193265171005.322.51E–73
    62Alpha tubulin (TcTUBA)gi|7139752549,767.5228061004.942.51E–74
    62Alpha tubulin (TcTUBA)gi|131420846,927.4207871005.491.99E–72
    63Beta tubulin 1.9 (TcTUBB)gi|1856813949,667.8166431004.745.00E–58
    64Beta tubulin of T. cyclops (TcTUBB)gi|9198319744,496.8146261005.682.51E–56
    65Calpain-like cysteine peptidase (TcCAPN1)gi|7140996212,839.351701005.081.26E–11
    66Tryparedoxin peroxidase family (TcGPX1)gi|1917115816,057.1510299.95.279.98E–51
    67Histone H2B of T. brucei (TbHIST1H2B)gi|7174843612,588.848496.111.771.26E–54
    68Beta tubulin (TcTUBB)gi|7165628149,667.7155701004.79.51E–56
    69Alpha tubulin of T. danilewskyi (TdTUBA)gi|7422992449,765.6217221004.946.29E–66
    70Cofilin-actin depolymerizing factor (TcCFL1)gi|7141769315,735.893001005.699.98E–24
    71Prostaglandin F synthase (TcPGFS)gi|7165136532,489.5165321007.716.29E–47
    72Hypothetical proteingi|71657351129,881.1187779.86.847.92E–41
    73Lipophosphoglycan biosynthetic protein (TcLPG3)gi|7166331386,968.5172481005.261.58E–18
    743-Hydroxy-3-methylglutaryl coenzyme A synthase-like (TcHMGCS2)gi|7166181654,719.2144451006.173.15E–38
    75Carboxypeptidase (TcCPD)gi|7166792957,626.9191361005.922.51E–07
Metallocarboxypeptidase (TcCPD)gi|4707607457,686.9181271005.851.99E–06
  • a T. cruzi epimastigotes (COL, SYL, and TCC isolates) were cultured in LIT media. Protein homogenates were resolved by two-dimensional gel electrophoresis. Gel images were analyzed on Progenesis SameSpots software, and normalized spot volumes were used to compare different groups. Proteins spots with a >2-fold change were subjected to MALDI-TOF MS/MS analysis. The number of peptides for each protein spot for which peptide mass fingerprint was obtained by MALDI-TOF MS is listed. Protein spots that were identified with high confidence by Mascot search engine (a protein score of >62) and statistically significant at the 95% confidence interval (CI%) are listed. The probability of match of the peptides in database search was accepted as significant at an E value of <0.001. pI, isoelectric pH. Underlined text indicates proteins involved in host cell invasion and cytoskeletal remodeling and motility. Boldface text indicates proteins of the antioxidant family involved in redox homeostasis.