TABLE 2

Protein spots that were differentially expressed in trypomastigote stage of TcI isolates of T. cruzia

Protein spotProtein name (gene)NCBI accession no.Mol mass (Da)Peptide countProtein scoreProtein score (CI%)pIE value
Spots differentially expressed in COL isolate compared to TCC
    1Tryparedoxin peroxidase (TcTXNPx)gi|1722495322,246.1103401005.961.99054E–28
    2Tryparedoxin peroxidase (TcTXNPx)gi|7139951422,375.1103041006.323.97164E–24
    5Calpain-like cysteine peptidase (Tc CL)gi|7141100612,798.351641005.077.92447E–11
    6Tryparedoxin peroxidase family (TcGPX1)gi|1917115816,057.148298.735.271.25674E–05
    7Paraflagellar rod protein 3 (TcPFR3)gi|222608468,705.3242881005.863.15479E–23
    8Paraflagellar rod protein, 69 kDa (TcPFR)gi|7165096369,518.9262151005.856.29463E–16
    9Paraflagellar rod protein 2 (TcPFR2)gi|16217969,504.9241981005.853.15479E–14
    10Paraflagellar rod protein of T. brucei (TbPFR-C/TbPFR-D)gi|7238719368,640.3191751005.786.29463E–12
    11Heat shock protein 70 (TcHSP70/TcHSPA5)gi|5065975671,329.9338771005.063.97164E–82
    12Glucose-regulated protein, 78 kDa (TcGRP78)gi|34983871,488296031005.079.97631E–55
    13Alpha tubulin (TcTUBA)gi|7139752549,767.5194051004.946.29463E–35
    14Alpha tubulin (TcTUBA)gi|131420846,927.4183981005.493.15479E–34
    15Beta tubulin of T. grayi (TgTUBB)gi|9198320142,806.9132881005.293.15479E–23
    16Beta tubulin of T. danilewskyi (TdTUBB)gi|7422992649,695.8132811004.741.58114E–22
    17Cytochrome c oxidase subunit IV (TcCOXIV)gi|7166785438,884.4153391005.722.50594E–28
    18Kinetoplastid membrane protein 11 (TcKMP11)gi|7141345510,876.383491005.731.25594E–28
    19Kinetoplastid membrane protein 11 of T. rangeli (TrKMP11)gi|3289204211,003.483471006.521.99054E–28
    20Phosphoglycerate kinase 1 (TcPGK1)gi|7141185944,441.1162401006.199.97631E–18
    213-Phosphoglycerate kinase, glycosomal (TcPGKC)gi|7165490054,867.6151221006.946.29463E–06
    22Chain A, tryparedoxin peroxidase (TcTXNPx)gi|5817694722,743.383051005.713.15479E–24
    23Peroxiredoxin, antioxidant oxidoreductase (TcPRX)gi|438865520,235.294401005.079.97631E–38
    24Ubiquitin precursor (TcUBQ)gi|16233740,105.654091009.391.25594E–34
    25Fe-superoxide dismutase, chain A (TcFeSODa)gi|15842865621,800.874411006.657.92447E–38
    26Fe-superoxide dismutase (TcFeSOD)gi|106633221,818.974401006.649.97631E–38
    27Calpain-like cysteine peptidase (TcCAPN1)gi|7140996212,839.341591005.081.25594E–09
    29Ascorbate-dependent peroxidase (TcAPX)gi|2437098231,855.272691006.691.25594E–20
Spots differentially expressed in SYL isolate compared to TCC
    30Beta tubulin of T. cyclops (TcTUBB)gi|9198319744,496.8142951005.686.29463E–24
    31Beta tubulin 1.9 (TcTUBB)gi|1856813949,667.8142911004.741.58114E–23
    32Prostaglandin F2 alpha synthase (TcPGFS)gi|7166116514,648.341191006.052.50594E–06
    33Amidinotransferase-like (TcGATM)gi|7166156743,051.9918410067.92447E–13
    34Amidinotransferase-like (TcGATM)gi|7141520228,667.451281005.213.15479E–07
    3510-kDa heat shock protein (TcHSPE1)gi|7142526310,692.826649.419.052.50594E–18
    36Tryparedoxin peroxidase, chain A (TcTXNPX)gi|5817694722,743.393281005.713.15479E–27
    37Alpha tubulin of T. danilewskyi (TdTUBAi)gi|7422992449,765.6183941004.947.92447E–34
    38Tubulin alpha (TcTUBA)gi|391508249,696.5173581004.93.15479E–30
    39NADPH flavin oxidoreductase (old yellow enzyme, TcOYE)gi|6174194242,191.6122501006.031.99054E–19
    40Glutamate dehydogenase (TcGLUD1)gi|7141498741,680.5112391007.12.50594E–18
    41Calmodulin (TcCALM1)gi|7140765826,740.25891005.13.15479E–17
    42Cysteine peptidase inhibitor (TcSERPIN)gi|7166469012,11428998.636.580.001256238
    43Ubiquitin homolog (TcUBQ)gi|1067314,648.854041009.823.97164E–34
    44Vacuolar ATP synthase subunit B-like (TcATP5B)gi|70870795104,474.5306561005.752.50594E–59
    47Heat shock protein 85 (TcHSP85)gi|7087649582,887.1186801005.799.97631E–62
    48Ubiquitin-like (TcUBQ)gi|1366778,503.654271006.561.99054E–36
    49Ubiquitin-like (TcUBQ)gi|106748,762.754251006.563.15479E–36
    50Kinetoplastid membrane protein 11 (TcKMP11)gi|61663889,923.983511006.587.92447E–29
    51Kinetoplastid membrane protein 11 of T. rangeli (TrKMP11)gi|2428654311,048.483491005.961.25594E–28
    52Cysteine-protease inhibitor/Chagasin (TcSERPIN)gi|11813789414,44831851006.663.15479E–12
    53Cysteine peptidase inhibitor (TcSERPIN)gi|7166469012,11421271006.581.99054E–06
    54Hypothetical protein (Tc CL)gi|7141063012,677.527123.036.581.99054E–12
    55Tryparedoxin peroxidase (TcTXNPX)gi|7141320722,376.184191005.691.25594E–35
    56Thioredoxin-dependent peroxide reductase (PRDX3)gi|24178583125,789.742141009.083.97164E–15
    57Iron superoxide dismutase (TcFeSOD)gi|7141437423,339.674391006.71.25594E–37
    58Iron superoxide dismutase B (TcFeSODB)gi|214961221,745.862851006.373.15479E–22
    59Heat shock 70-kDa protein (TcHSP70)gi|7140630440,831187201006.199.97631E–66
    60Heat shock protein HSP70 (TcHSP70)gi|16211771,210.1196851005.423.15479E–62
    61Heat shock protein 70 (TcHSP70)gi|1062674,233.7176091005.641.25594E–54
    62Ubiquitin-conjugating enzyme-like (Tc CL)gi|7141404215,754.891571005.811.99054E–09
    63Beta tubulin 1.9 (TcTUBB)gi|1856813949,667.8122271004.741.99054E–16
    64Beta tubulin (TcTUBB)gi|391588349,540.7112621004.691.25594E–20
    65Alpha tubulin (TcTUBA)gi|131420846,927.4184001005.499.97631E–34
    66Alpha tubulin (TcTUBA)gi|7139752549,767.5183961004.942.50594E–33
    67Calpain-like cysteine peptidase (TcCAPN1)gi|7141100612,798.351771005.071.99054E–11
    683-Phosphoglycerate kinase (TcPGK)gi|1694552754,767.4151211006.947.92447E–06
    69Phosphoglycerate kinase (TcPGKCL)gi|7141186264,528.8141141008.043.97164E–05
    70Tryparedoxin peroxidase (TcTXNPX)gi|1722495322,246.194921005.966.29463E–43
    71Cruzipaingi|113630847,852.133481005.351.58114E–28
    7269-kDa paraflagellar rod protein (TcPFR)gi|7141138742,471.9181531006.129.97631E–10
    73Surface glycoprotein, 82 kDa (TcGP82)gi|604914859,591.9201701006.059.97631E–11
  • a T. cruzi trypomastigotes (COL, SYL, and TCC isolates) were cultured in C2C12 cells. Parasite protein homogenates were resolved by two-dimensional gel electrophoresis. Gel images were analyzed on Progenesis SameSpots software, and normalized spot volumes were used for comparison of different groups. Proteins spots with >2-fold change were subjected to MALDI-TOF MS/MS analysis. The significance of a protein match, based on the peptide mass fingerprint in the MS and the MS/MS data, is presented as expectation values (P < 0.01). pI, isoelectric pH. Underlined text indicates proteins involved in host cell invasion and cytoskeletal remodeling and motility. Boldface text indicates proteins of the antioxidant family that are involved in redox homeostasis.