TABLE 1

Bacterial strains used in this study

StrainRelevant featuresaReference or source
B. mallei ATCC 23344Human clinical isolate; Kms Pbr26
B. mallei CSM001B. mallei ATCC 23344 with mini-Tn5::luxKm2; Kmr Pbr27
B. mallei TMM001B. mallei ATCC 23344 with an unmarked intragenic deletion in bmaa1801tonB)16
B. mallei CLH001B. mallei TMM001 with unmarked intragenic deletion in bmaa0742hcp1)This study
B. mallei CLH002B. mallei ATCC 23344 with an unmarked intragenic deletion of bmaa0742hcp1)This study
E. coli S17-1 (pMo130-ΔBPSS1498)Donor strain containing pMo130–Δbpss1498 plasmid; Smr Tpr Pbs Kmr22
  • a Km, kanamycin; Pb, polymyxin B; Sm, streptomycin; Tp, trimethoprim.