TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidDescription
Strains
    K. pneumoniae
        NTUH K-2044 (WT)Wild-type strain; K1 serotype; Apr
        ΔfrwCDeletion of frwC from WT; Apr
        C-frwCComplemented frwC mutant; Apr Kmr
        ΔcrpDeletion of crp from WT; Apr
    E. coli
        DH5αλ φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA1
Plasmids
    pKO3-KmpKO3-derived plasmid with insertion of a Km resistance cassette from pUC4K into the AccI site
    pGEM-T-easypGEM-T easy with insertion of a Km cassette from pUC4K into the NdeI site for trans-complementation
    pHRP309lacZ transcriptional fusion vector based on the IncQ plasmid RSF1010