TABLE 1

Strains used in this study

Organism and strain or plasmidRelevant genotypeaReference
Escherichia coli
    S17-1λpirRP4-2 Tc::Mu-Km::Tn7pir)51
Yersinia pseudotuberculosis
    YPIII pIB102yadA, Kmr (wild type)12
    YPIII pIB19pIB102: lcrV, Kmr52
    YPIII pIB604pIB102: yopB, Kmr53
    YPIII pIB526pIB102: yopE, Kmr54
    YPIII pIB822pIB102: yopN full-length in-frame deletion; KmrThis study
    YPIII pIB822604pIB822: yopB, KmrThis study
    YPIII pIB102 YopH-BlapIB102: YopH99-Bla24–286, Kmr, Cmlr35
    YPIII pIB822 YopH-BlapIB822 expressing YopH99-Bla24–286, Kmr, CmlrThis study
    YPIII pIB822604 YopH-BlapIB822604 expressing YopH99-Bla24–286, Kmr, CmlrThis study
    YPIII pIB102 YopE-BlapIB102: YopE86-Bla24–286, Kmr, Cmlr35
    YPIII pIB822 YopE-BlapIB822 expressing YopE86-Bla24–286, Kmr, CmlrThis study
    YPIII pIB822604 YopE-BlapIB822604 expressing YopE86-Bla24–286, Kmr, CmlrThis study
Plasmids
    pNQ705-H-BlapNQ705 containing YopH6–99-β-lactamase fusion35
    pNQ705-E-BlapNQ705 containing YopE6–86-β-lactamase fusion35
    pSE822pDM4 yopN in-frame deletion of aa 9–272This study
    pSE1pBAD24 YopNwtThis study
    pSB1pBAD24 YopNwt-HAThis study
    pRN62pBAD24 YopN76-181-HAThis study
    pRN63pBAD24 YopN131-168-HAThis study
    pRN64pBAD24 YopN170-207-HAThis study
  • a Km, kanamycin; Cml, chloramphenicol.