TABLE 2

Oligonucleotides used in this study

Primer purpose and gene or plasmidDirection and useaSequenceReference
qPCR
    hilAFwdATAGCAAACTCCCGACGATG79
RevATTAAGGCGACAGAGCTGG
    hilDFwdGGTAGTTAACGTGACGCTTG79
RevGATCTTCTGCGCTTTCTCTG
    rtsAFwdACCCGTGGTGAGCTTGATGAGT79
RevCCTGTCCAGGTGGGGAGCAT
    sicPFwdAGATGATATCTGGTTATTGAACGGTATG79
RevCTGCCGCCAGATAGAATCG
    hilCFwdCTCACCTCTTCAGCGGCCAGT79
RevCACCCGCAAATGGTCACAGGCT
    prgHFwdTGAACGGCTGTGAGTTTCCA79
RevGCGCATCACTCTGACCTACCA
    sipBFwdGGCGCGCTGCTAACCAT79
RevTCGCCCCACCGGTAAAA
    invFFwdTGTCGCACCAGTATCAGGAG80
RevAAATAGCGCGAAACTCAGGA
    hilEFwdAAAGCCGGATCAAAGGTTTT80
RevCTTTCACCGTTTTCCCGTTA
    flhDFwdTGTTCCGCCTCGGTATCAAC81
RevCGCGAATCCTGAGTCAAACG
    fliAFwdGATTGAATCGCTGCCGGAAC81
RevACTATGCAACTGGCTGACCC
    fliZFwdAAACATTTCCCACGATCTGC81
RevCGGTAAAGGGGGATTTCTG
    rrsFwdCGGGGAGGAAGGTGTTGTG80
RevCAGCCCGGGGATTTCACATC
Bacteriophage lambda Red deletion
    metJFwd cassette generationGGGCTCAGGTTCAGACCTCAATATTAATGACGAAGAGGATTAAGTATCTCGTGTAGGCTGGAGCTGCTTC
Rev cassette generationGAATCGTTAAAAAAGCGCGGCCAGAGGCGTTCTGACCGCATGCTTTGCTACATATGAATATCCTCCTTAG
UpstreamCATCTGCGACCGCTAACTT
DownstreamTTTATCCACCGAGGGTTATTCG
    fliZFwd cassette generationCGAAAAGTGCCGCACAACGTATAGACTACCAGGAGTTCTCGTGTAGGCTGGAGCTGCTTC
Rev cassette generationCACGTTTCACCAACACGACTCTGCTACATCTTATGCTTTTCATATGAATATCCTCCTTAG
UpstreamCATCGAACTGGTGACTGAAGA
DownstreamCTACAGCCATTACTCCCATCAG
    flhDCFwd cassette generationGTGCGGCTACGTCGCACAAAAATAAAGTTGGTTATTCTGGGTGTAGGCTGGAGCTGCTTC
Rev cassette generationATGACTTACCGCTGCTGGAGTGTTTGTCCACACCGTTTCGCATATGAATATCCTCCTTAG
UpstreamCGAGTAGAGTTGCGTCGAATTA
DownstreamATCCTTCCGCTGTTGACTATG
    hilDFwd cassette generationCCAGTAAGGAACATTAAAATAACATCAACAAAGGGATAATGTGTAGGCTGGAGCTGCTTC
Rev cassette generationTTAATAAAAATCTTTACTTAAGTGACAGATACAAAAAATGCATATGAATATCCTCCTTAG
UpstreamCTGGGCTTGTTATCGTCTTCT
DownstreamCAGGAGGGTTATGAGCAGTATC
pKD46FwdCAGTCATAGCCGAATAGCCT75
pKD46RevCGGCCACAGTCGATGAATCC75
  • a Fwd, forward; Rev, reverse.