TABLE 1

Immunogenic epitopes identified by peptide screens of mycobacterial ribosomal proteinsa

PeptideResiduesSequenceP value
Rv0652 (RplL)
    793–107AKDLVDGAPKPLLEK0.0022
    12791–105KEAKDLVDSAPKPLL0.0001
Rv2904 (RplS)
    1069–83VERTFPVHSPNIDHI0.0001
    1115–29DIPAFNPGDTINVHV0.0290
    1265–79YGVGVERTFPVHSPN0.0240
    1373–87FPVHSPNIDHIEVVT0.0002
Rv0707 (RpsC)
    16210–224GKRELAAAAPAGADR0.0240
    20206–220DIVGGKRELAAAAPA0.0167
    32182–196IDYGLYEAKTTFGRI0.0240
Rv0640 (RpIK)
    4312–26KLQIVAGQANPAPPV0.0025
    4467–81SFTFTLKTPPAAKLL0.0290
    4516–30VAGQANPAPPVGPAL0.0167
Rv0651 (RpIJ)
    123146–160KAAGLFNAPASQVAR0.0001
    124151–165FNAPASQVARLAAAL0.0001
Rv0683 (RpsG)
    5616–30PVYGSQLVTQLVNKV0.0188
Rv0700 (RpsJ)
    6532–46TGASVVGPVPLPTEK0.0006
    6728–42TVVRTGASVVGPVPL0.0401
    17231–45RTGASVVGPVPLPTE0.0001
Rv0682 (RpsL)
    10218–32KVKTAALKGSPQRRG0.0458
    10330–44RRGVCTRVYTTTPKK0.0240
Rv1630 (RpsA)
    154431–445QMEKFAAAEAEAANA0.0001
Rv3458c (RpsD)
    1621–15MARYTGPATRKSRRL0.0018
  • a Peptides identified in screens for immunogenic epitopes (Fig. 2 and 3) are listed with their locus tag (name), library identification, peptide number, residue location in the intact protein, amino acid sequence, and statistical significance in the screen conducted. Proteins identified as being immunogenic using the approach illustrated in Fig. 1 are highlighted in boldface type.