Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aSource or reference
    A. baumannii
        ATCC 17978Clinical isolateATCC
        17978.ORblsA::aph derivative of ATCC 17978; Kmr24
        17978.prpAprpA::ermAM derivative of ATCC 17978; EmrThis work
        17978.prpA.EVprpA mutant harboring pWH1266; Emr TetrThis work
        17978.prpA.CVprpA mutant harboring pMU1269; Ampr Emr TetsThis work
    E. coli
        DH5αDNA recombinant methodsGibco-BRL
        Top10DNA recombinant methodsLife Technologies
    Lactococcus lactis subsp. cremorisStrain MG1363 harboring pIL252; EmrH. Smidt and T. Kruse
    pCR8-TOPOGateway cloning vector; SprLife Technologies
    pEX100TSuicide vector for allelic exchange; AmprATCC
    pIL252Cloning vector; source of ermAM encoding EmrH. Smidt and T. Kruse
    pWH1266A. baumannii-E. coli shuttle vector; Ampr Tetr42
    pMU1135pCR8-TOPO harboring prpA; SprThis work
    pMU1142pCR8-TOPO harboring prpA::ermAM; Spr EmrThis work
    pMU1143pEX100T harboring prpA::ermAM; Ampr EmrThis work
    pMU1269pWH1266 harboring prpA; Ampr TetsThis work
  • a Ampr, ampicillin resistance; Emr, erythromycin resistance; Kmr, kanamycin resistance; Spr, spectinomycin resistance; Tetr, tetracycline resistance; Tets, tetracycline sensitivity.